Machine Learning Discoveries of RHNO1-X Synergy in ETC-1922159 Treated Colorectal Cancer Cells

In response to DNA damage and replication stress, RHINO RAD9-HUS1-RAD1 interacting nuclear orphan (RHNO1), interacts with RAD9-HUS1-RAD1 (9-1-1) clamp and TOPBP1, to activate ATR signaling pathway. Recently, it has been found to be implicated in cancer as it is often overexpressed. In colorectal cancer (CRC) cells treated with ETC-1922159, RHNO1 was found to be down regulated along with other genes. A recently developed search engine ranked combinations of RHNO1-X (X, a particular gene/protein) at 2nd order level after drug administration. Some of these combinations have been tested in wet lab, however many have been pointed out by the search engine that are yet to be explored/tested. These rankings reveal which RHNO1-X combina- tions might be working synergistically in CRC. In this research work, I cover combinations of RHNO1 with possible members of DNA topoisomerase (TOP), nei like DNA glycosylase (NEIL), flap structure-specific endonuclease 1 (FEN1), tumor protein p53 (TP53), ATR serine/threonine kinase (ATR), cell division cycle (CDC), forkhead box (FOX) and bone morphogenetic protein (BMP) family.