Machine Learning Discoveries of TOP2A-X Synergy in ETC-1922159 Treated Colorectal Cancer Cells

DNA topoisomerase II α (TOP2A) belongs to the family of topoisomerases, which regulates the (un)winding of the DNA due to its double helical structure. The main function of TOP2A is to relieve the topological stress during DNA transcription, assist in separation of chromatids and condensation of chromosomes. In colorectal cancer (CRC) cells treated with ETC-1922159, TOP2A was found to be down regulated along with other genes. A recently developed search engine ranked combinations of TOP2A-X (X, a particular gene/protein) at 2nd order level after drug administration. Some of these combinations have been tested in wet lab, however many have been pointed out by the search engine that are yet to be explored/tested. These rankings reveal which TOP2A-X combinations might be working synergistically in CRC. In this research work, I cover combinations of TOP2A with WNT, nucleolar and spindle associated protein (NUSAP), Wolf-Hirschhorn syndrome candidate (WHSC), rhophilin Rho GTPase binding protein antisense RNA (RHPN-AS1), AT-rich interaction do- main (ARID), DNA topoisomerase II binding protein (TOPBP), ERCC excision repair (ERCC), enhancer of zeste polycomb repressive complex 2 subunit (EZH), cyclin dependent kinase (CDK), origin recognition complex subunit (ORC), interleukin (IL), ubiquitin specific peptidase (USP), RAD54, zinc finger protein (ZNF), high mobility group box (HMGB), E2F transcription factor (E2F), GINS complex subunit (GINS), minichromosome maintenance (MCM), budding uninhibited by benzimidazoles mitotic checkpoint (BUB), DEAD/H-box helicase (DDX), H2A histone family member (H2A) and structural maintenance of chromosomes (SMC) family.