Machine Learning Discoveries of FANCD2-X Synergy in etc-1922159 Treated Colorectal Cancer Cells

Fanconi anemia complementation group D2 (FANCD2) is one of the members of FA complementation group, that is activated in response to DNA damage and is involved in DNA repair and regulation of genomic stability. FANCD2 works with other members of FANC group along with other proteins/genes, to carry out its required functionality. Further, disruption of FA/BRCA pathway has been implicated in cancer progression. In colorectal cancer (CRC) cells treated with ETC-1922159, FANCD2 was found to be down regulated along with other genes. A recently developed search engine ranked combinations of FANCD2-X (X, a particular gene/protein) at 2nd order level after drug administration. Some of these combinations have been tested and established in wet lab, however many have been pointed out by the search engine that are yet to be explored/tested. These rankings reveal which FANCD2-X combinations might be working synergistically in CRC. In this research work, I cover combinations of FANCD2 with, REV3 like DNA directed polymerase zeta catalytic subunit (REV3L), Bloom syndrome RecQ like helicase (BLM), MRE11 homolog double strand break repair nuclease (MRE11A), Wnt family member 10B (WNT10B), ubiquitin like with PHD and ring finger domains 1 (UHRF1), hes family bHLH transcription factor 1 (HES1), BRCA DNA repair associated (BRCA), RAD51 recombinase (RAD51), ERCC excision repair endonuclease non-catalytic subunit (ERCC), KIAA, X-ray repair cross com- plementing (XRCC), structural maintenance of chromosomes (SMC), WD repeat do- main containing (WDR), ubiquitin conjugating enzyme E2 (UBE2), cell division cycle (CDC), importin (IPO), aldehyde dehydrogenase family member (ALDH), H2A variant histone (H2A), heat shock protein (HSP), cyclin dependent kinase (CDK), dynein axonemal heavy chain (DNAH), forkhead box (FOX), ring finger protein (RNF), E2F transcription factor (E2F) and small nucleolar RNA host gene (SNHG) family.