A low-cost FPGA-based approach for pile-up corrected high-speed in vivo FLIM imaging
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Despite these advantages, the widespread adoption of FLIM has been constrained by the lack of straightforward approaches to correct pile-up distortions that would otherwise limit its application for capturing biological dynamics on second and sub-second timescales.
I found your work on LPBT correction for pile-up distortions very impressive. I'm curious if you could propose a straightforward experiment to determine whether a commercial FLIM system is significantly affected by pile-up distortions? Perhaps using your fluorescein with potassium iodide approach as a standardized test?
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