Light-Dependent Ethanol Assimilation By Model Red Microaga Galdieria sulphuraria
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Abstract
The effect of ethanol on the red thermoacidophilic microalga Galdieria sulphuraria , capable of hemo- and photoheterotrophic growth, was studied. It was found that G. sulphuraria failed to grow and assimilate ethanol when kept in the dark, in contrast to a wide range of organic compounds known to this alga. Light conditions, on the other hand, activated ethanol uptake from the growth medium and intensify microalgal growth. The known stress effect of ethyl alcohol can be seen in the short-term increase in cellular respiration in the dark and its sustained increase throughout the growth period of G. sulphuraria in the light. Ethanol-induced increase in cellular respiration precedes light activation of photosynthesis. The growth enhancement of ethanol-supplemented culture of G. sulphuraria compared to photoautotrophic culture is most likely due to the complete oxidation of ethanol during respiration to carbon dioxide and its use by chloroplasts as an additional intracellular substrate for photosynthesis. The absence of ethanol consumption in the dark and its metabolic uptake in the light indicate photoactivation of alcohol dehydrogenase and acetaldehyde dehydrogenase, two key sequential enzymes in the ethanol oxidation pathway.
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Microaga
Spelling Error in the title
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. For dark autotrophic cells of G. sulphuraria, an almost twofold smooth decrease in the rate of O2 uptake on the 4-th day of cultivation is evident due to depletion of respiratory substrates in the absence of photosynthesis (Fig. 5).
ok, so the increased oxygen uptake in day two with ethanol is not because of increased cell growth since the ethanol didn't impact cell density in the dark, right? very interesting!
This is very neat work! Thank you for sharing it
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G
This is a very convincing figure and consistent with the increased cell density at day 4 when treated with ethanol! Very cool!
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Thus, the non-usage of ethanol by G. sulphuraria cells in the dark and the consumption of ethyl alcohol from the culture medium in the light correlate with the data on additional light-induced cell growth
did y'all do any statistics on this ethanol loss dataset?
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. Microscopic control showed that the cells in both dark cultures remained viable, i.e. in a state of survival, throughout the duration of the experiment.
Where there any notable morphology phenotypes observed? Also, I didn't see any mention of microscopy in the methods. What sort of imaging did you do?
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No statistically significant changes in growth were also observed in the dark in the presence of ethanol, as opposed to light
So there was no statistical difference in the growth rate of normal cultures in light vs ethanol-treated cultures in the dark? The abstract/intro seemed to say that ethanol had no impact on dark cultures, correct? So there is no statistical difference in the growth rate of G. sulphuraria in normal media in the light vs normal media in the dark?
Is the legend saying Curve 3 represents dark cultures in normal media AND dark cultures with ethanol? If not, why is there no curve for the dark growth WITHOUT ethanol?
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rowth
minor spelling error
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disoder
minor spelling error
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