Raman flow cytometry using time delay integration
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Abstract
Raman flow cytometry offers chemically sensitive, label-free measurement of cells and particles; however, the technique suffers from low cell throughput due to the weak Raman signal. Here, we demonstrate the use of time-delay integration to achieve Raman flow cytometry combined with dual-sided line illumination. The use of line illumination from both sides of the cell flow capillary kept the cell stream in the detection area by balancing optical force from the illumination lines. The time-delay integration allowed accumulation of Raman signals from flowing cells without sacrificing the spectrum readout rate. With the developed system, we achieved Raman flow cytometry at throughputs of 32 and 75 events per second for cell and particle detection, respectively. We applied the technique for analyzing lipid uptake in HepG2 cells and degranulation in bone marrow-derived murine mast cells.
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acoustic focusing from an attached pi-ezoelectric transducer to confine cell motion to the center ofthe capillary
a citation describing acoustic focusing may be useful. is it known how well the particles are confined using this technique?
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Conclusion
very impressive technology!
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-865, nac Image Tech-nology
i may have missed it but what is the CMOS camera used for in the study?
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depending laser out-put
typo: depending on laser output
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0.26 – 3.2 mW/μm
this seems like a large amount of power, is it possible to theoretically determine the temperature increase that this light could induce in a transiting object?
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