MLCK/MLCP regulates mammalian axon regeneration via the redistribution of the growth cone F-actin
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Saijilafu et al. describe that MLCK and MLCP bidirectionally regulate NMII phosphorylation ultimately impinging on axonal growth during regeneration in the central and peripheral nervous systems. However, the evidence is in most cases incomplete, since some key controls are missing, some major claims are too broad to be supported by data and some claims and evidence present internal contradictions. In sum, this knowledge is potentially useful for the field due to the relevance of identifying mechanisms that regulate axonal regeneration, providing some claims inconsistencies are better supported and properly discussed.
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Abstract
Axon regrowth is a key determinant of the restoration of the biological function of the nervous system after trauma. However, mature mammalian neurons have limited capacity for axon regeneration. We have previously demonstrated that neuronal axon growth both in the central and the peripheral nervous systems is markedly enhanced when non-muscle myosin II (NMII) is inhibited with blebbistatin. The activity of NMII is primarily regulated by MLCK and MLCP via the phosphorylation and dephosphorylation of its light chain, respectively; however, the functional roles of MLCK and MLCP in mammalian axonal regeneration remain unknown. In the present study, we provide strong evidence that the inhibition of MLCK activity significantly blocks axon regeneration in the mouse. Conversely, inhibition of MLCP promotes axon regrowth of both peripheral and central nervous system. Our findings further indicate that the MLCK/MLCP regulates axon regeneration via the reorganization of F-actin distribution in the growth cone, and this result suggests that direct regulation of the growth cone machinery is a potential strategy to promote axon regeneration.
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eLife assessment
Saijilafu et al. describe that MLCK and MLCP bidirectionally regulate NMII phosphorylation ultimately impinging on axonal growth during regeneration in the central and peripheral nervous systems. However, the evidence is in most cases incomplete, since some key controls are missing, some major claims are too broad to be supported by data and some claims and evidence present internal contradictions. In sum, this knowledge is potentially useful for the field due to the relevance of identifying mechanisms that regulate axonal regeneration, providing some claims inconsistencies are better supported and properly discussed.
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Reviewer #1 (Public review):
This paper examines the role of MLCK (myosin light chain kinase) and MLCP (myosin light chain phosphatase) in axon regeneration. Using loss-of-function approaches based on small molecule inhibitors and siRNA knockdown, the authors explore axon regeneration in cell culture and in animal models. Their evidence shows that MLCK activity facilitates axon extension/regeneration, while MLCP prevents it.
Major concern:
A global inconsistency in the conclusions of the authors is evident when trying to understand the role of NMII in axon growth and to understand the present results in light of previous reports by the authors and many others on the role of NMII in axon extension. The discussion of the matter fails to acknowledge a vast literature on how NMII activity is regulated. The authors study enzymes responsible …
Reviewer #1 (Public review):
This paper examines the role of MLCK (myosin light chain kinase) and MLCP (myosin light chain phosphatase) in axon regeneration. Using loss-of-function approaches based on small molecule inhibitors and siRNA knockdown, the authors explore axon regeneration in cell culture and in animal models. Their evidence shows that MLCK activity facilitates axon extension/regeneration, while MLCP prevents it.
Major concern:
A global inconsistency in the conclusions of the authors is evident when trying to understand the role of NMII in axon growth and to understand the present results in light of previous reports by the authors and many others on the role of NMII in axon extension. The discussion of the matter fails to acknowledge a vast literature on how NMII activity is regulated. The authors study enzymes responsible for the phosphorylation and dephosphorylation of NMII, referring to something that is strongly proven elsewhere, that phosphorylation activates NMII and dephosphorylation deactivates it. The authors mention their own previous evidence using inhibitors of NMII ATPase activity (blebbistatin, Bleb for short) and inhibitors of a kinase that phosphorylates NMII (ROCK), highlighting that Bleb increases axon growth. Since Bleb inhibits the ATPase activity of NMII, it follows that NMII is in itself an inhibitor of axon growth, and hence when NMII is inhibited, the inhibition on axon growth is relieved, and axonal growth takes place (REF1). It is known that NMII exists in an inactive folded state, and ser19 phosphorylation (by MLCK or ROCK) extends the protein, allowing NMII filament formation, ATPase activity, and force generation on actin filaments (REF2). From this, it is derived that if MLCK is inhibited, then there is no NMII phosphorylation, and hence no NMII activity, and, according to their previous work, this should promote axon growth. On the contrary, the authors show the opposite effect: in the lack of phospho-MLC, authors show axon growth inhibition.
Reporting evidence challenging previous conclusions is common business in scientific endeavors, but the problem with the current manuscript is that it fails to point to and appropriately discuss this contradiction. Instead, the authors refer to the fact that MLCK and Bleb inhibit NMII in different steps of the activation process. While this is true, this explanation does not solve the contradiction. There are many options to accommodate the information, but it is not the purpose of this revision to provide them. Since the manuscript is focused solely on phosphorylation states of MLC and axon extension, the claims are simply at odds with the current literature, and this important finding, if true, is not properly discussed.
What follows is a discussion of the merits and limitations of different claims of the manuscript in light of the evidence presented.
(1) Using western blot and immunohistochemical analyses, authors first show that MLCK expression is increased in DRG sensory neurons following peripheral axotomy, concomitant to an increase in MLC phosphorylation, suggesting a causal effect (Figure 1). The authors claim that it is common that axon growth-promoting genes are upregulated. It would have been interesting at this point to study in this scenario the regulation of MLCP, which is a main subject in this work, and expect its downregulation.
(2) Using DRG cultures and sciatic nerve crush in the context of MLCK inhibition and down-regulation, authors conclude that MLCK activity is required for mammalian peripheral axon regeneration both in vitro and in vivo (Figure 2).
The in vitro evidence is of standard methods and convincing. However, here, as well as in all other experiments using siRNAs, it is not clear what the control is about (the identity of the plasmids and sequences, if any).
Related to this, it is not helpful to show the same exact picture as a control example in Figures 2 and 3 (panels J and E, respectively). Either because they should not have received the same control treatment, or simply because it raises concern that there are no other control examples worth showing. In these images, it is not also clear where and how the crush site is determined in the GFP channel. This is of major importance since the axonal length is measured from the presumed crush site. Apart from providing further details in the text, the authors should include convincing images.
(3) The authors then examined the role of the phosphatase MLCP in axon growth during regeneration. The authors first use a known MLCP blocker, phorbol 12,13-dibutyrate (PDBu), to show that is able to increase the levels of p-MLC, with a concomitant increase in the extent of axon regrowth of DRG neurons, both in permissive as well as non-permissive. The authors repeat the experiments using the knockdown of MYPT1, a key component of the MLC-phosphatase, and again can observe a growth-promoting effect (Figure 3).
The authors further show evidence for the growth-enhancing effect in vivo, in nerve crush experiments. The evidence in vivo deserves more evidence and experimental details (see comment 2). Some key weaknesses of the data were mentioned previously (unclear RNAi controls and duplication of shown images), but in this case, it is also not clear if there is a change only in the extent of growth, or also in the number of axons that are able to regenerate.
(4) In the next set of experiments (presented in Figure 4) authors extend the previous observations in primary cultures from the CNS. For that, they use cortical and hippocampal cultures, and pharmacological and genetic loss-of-function using the above-mentioned strategies. The expected results were obtained in both CNS neurons: inhibition or knockdown of the kinase decreases axon growth, whereas inhibition or knockdown of the phosphatase increases growth. A main weakness in this set is that it is not indicated when (at what day in vitro, DIV) the treatments are performed. This is important to correctly interpret the results, since in the first days in vitro these neurons follow well-characterized stages of development, with characteristic cellular events with relevance to what is being evaluated. Importantly, this would be of value to understand whether the treatments affect axonal specification and/or axonal extension. Although these events are correlated, they imply a different set of molecular events.
The title of this section is misleading: line 241 "MLCK/MLCP activity regulated axon growth in the embryonic CNS"... the title (and the conclusion) implies that the experiments were performed in situ, looking at axons in the developing brain. The most accurate title and conclusion should mention that the evidence was collected in CNS primary cultures derived from embryos.
(5) Performing nerve crush injury in CNS nerves (optic nerve and spinal cord), and the local application of PBDu, the author shows contrasting results (Figure 5). In the ON nerve, they can see axons extending beyond the lesion site due to PBDu. On the contrary, the authors fail to observe so in the corticospinal tract present in the spinal cord. The authors fail to discuss this matter in detail. Also, they accommodate the interpretation of the evidence in light of a process known as axon retraction, and its prevention by MLCP inhibition. Since the whole paper is on axon extension, and it is known that mechanistically axon retraction is not merely the opposite of axon extension, the claim needs far more evidence.
In panel 5F and the supplementary data, the authors mention the occurrence of retraction bulbs, but the images are too small to support the claim, and it is not clear how these numbers were normalized to the number of axons labeled in each condition.
(6) The author combines MLCK and MLCP inhibitors with Bleb, trying to verify if both pairs of inhibitors act on the same target/pathway (Figure 6). The rationale is wrong for at least two reasons.
a- Because both lines of evidence point to contrasting actions of NMII on axon growth, one approach could never "rescue" the other.
b- Because the approaches target different steps on NMII activation, one could never "prevent" or rescue the other. For example, for Bleb to provide a phenotype, it should find any p-MLC, because it is only that form of MLC that is capable of inhibiting its ATPase site. In light of this, it is not surprising that Bleb is unable to exert any action in a situation where there is no p-MLC (ML-7, which by inhibiting the kinase drives the levels of p-MLC to zero, Figure 4A). Hence, the results are not possible to validate in the current general interpretation of the authors. (See 'major concern').(7) In Figure 7, the authors argue that the scheme of replating and using ML7 before or after replating is evidence for a local cytoskeletal action of the drug. However, an alternative simpler explanation is that the drug acts acutely on its target, and that, as such, does not "survive" the replating procedure. Hence, the conclusion raised by the evidence shown is not supported.
(8) In Figure 8, the authors show that the inhibitory treatments on MLCK and MLCP (ML7 and PRBu) alter the morphology of growth cones. However, it is not clear how this is correlated with axon growth. The authors also mention in various parts of the text that a local change in the growth cone is evidence for a local action/activity of the drug or enzyme. However the local change<->local action is not a logical truth. It can well be that MLCK and MLCP activity trigger molecular events that ultimately have an effect elsewhere, and by looking at "elsewhere" one observes of course a local effect, but is not because the direct action of MLCK or MLCP are localized. To prove true localized effects there are numerous efforts that can be made, starting from live imaging, fluorescent sensors, and compartmentalized cultures, just to mention a few.
References:
(1) Eun-Mi Hur 1, In Hong Yang, Deok-Ho Kim, Justin Byun, Saijilafu, Wen-Lin Xu, Philip R Nicovich, Raymond Cheong, Andre Levchenko, Nitish Thakor, Feng-Quan Zhou. 2011. Engineering neuronal growth cones to promote axon regeneration over inhibitory molecules. Proc Natl Acad Sci U S A. 2011 Mar 22;108(12):5057-62. doi: 10.1073/pnas.1011258108.
(2) Garrido-Casado M, Asensio-Juárez G, Talayero VC, Vicente-Manzanares M. 2024. Engines of change: Nonmuscle myosin II in mechanobiology. Curr Opin Cell Biol. 2024 Apr;87:102344. doi: 10.1016/j.ceb.2024.102344.
(3) Karen A Newell-Litwa 1, Rick Horwitz 2, Marcelo L Lamers. 2015. Non-muscle myosin II in disease: mechanisms and therapeutic opportunities. Dis Model Mech. 2015 Dec;8(12):1495-515. doi: 10.1242/dmm.022103.
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Reviewer #2 (Public review):
Summary:
Saijilafu et al. demonstrate that MLCK/MLCP proteins promote axonal regeneration in both the central nervous system (CNS) and peripheral nervous system (PNS) using primary cultures of adult DRG neurons, hippocampal and cortical neurons, as well as in vivo experiments involving sciatic nerve injury, spinal cord injury, and optic nerve crush. The authors show that axon regrowth is possible across different contexts through genetic and pharmacological manipulation of these proteins. Additionally, they propose that MLCK/MLCP may regulate F-actin reorganization in the growth cone, which is significant as it suggests a novel strategy for promoting axonal regeneration.
Strengths:
This manuscript presents a comprehensive array of experimental models, addressing the biological question in a broad manner. …
Reviewer #2 (Public review):
Summary:
Saijilafu et al. demonstrate that MLCK/MLCP proteins promote axonal regeneration in both the central nervous system (CNS) and peripheral nervous system (PNS) using primary cultures of adult DRG neurons, hippocampal and cortical neurons, as well as in vivo experiments involving sciatic nerve injury, spinal cord injury, and optic nerve crush. The authors show that axon regrowth is possible across different contexts through genetic and pharmacological manipulation of these proteins. Additionally, they propose that MLCK/MLCP may regulate F-actin reorganization in the growth cone, which is significant as it suggests a novel strategy for promoting axonal regeneration.
Strengths:
This manuscript presents a comprehensive array of experimental models, addressing the biological question in a broad manner. Particularly noteworthy is the use of multiple in vivo models, which significantly strengthens the overall validity of the study.
Weaknesses:
The following aspects apply:
(1) The manuscript initially references prior research by the authors suggesting that NMII inhibition enhances axonal growth and that MLCK activates NMII. However, the study introduces a contradiction by demonstrating that MLCK inhibition (via ML-7 or siMLCK) inhibits axonal growth. This inconsistency is not adequately addressed or discussed in the manuscript.
(2) While the study proposes that MLCK/MLCP regulates F-actin redistribution in the growth cone, the mechanism is not explored in depth. The only figure showing how pharmacological manipulation affects the growth cone suggests that not only F-actin but also the microtubule cytoskeleton might be affected, indicating that the mechanism may not be specific. A deeper exploration of this relationship in DRG neurons, in addition to cortical neurons, as shown in the study, would be beneficial.
(3) In the sciatic nerve injury experiments, it would be crucial to include additional controls that clearly demonstrate that siMYPT1 treatment increases MLCP in the L4-L5 ganglia. Additionally, although the manuscript mentions quantifying axons expressing EGFP, the Materials and Methods section only discusses siMYPT1 electroporation, which could lead to confusion.
(4) In some panels, it is difficult to differentiate the somas from the background (Figures 3, 4, 7). In conditions where images with shorter axonal lengths are represented, it is unclear whether this is due to fewer cells or reduced axonal growth (Figures 2, 4, 6).
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