Optimizing Protein Production in the One-Pot PURE System: Insights into Reaction Composition and Expression Efficiency
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Abstract
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lifetime
It would be very interesting to know the chemical composition differences between the two energy mixes. Is this proprietary? Do you have any speculation?
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One-Pot PURE proteins
Do you have any speculations on why this might be? What do you think the rate limiting component is, then?
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PURE system
There does not appear to be any reference in the text to figure 3A-B. In addition, it would be nice to clarify the difference between 'pure A' and 'pure B' earlier on in the text! This also makes the figure confusing.
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1% w/v glucose
That's a very simple fix! Very cool to see.
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glycerol stocks
Was this a reproducible loss? Was it always the same ten proteins?
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