Synthetic Type III-E CRISPR-Cas Effectors for Programmable RNA-targeting

  1. Daniel J. Brogan
  2. Calvin P. Lin
  3. Elena Dalla Benetta
  4. Tianqi Wang
  5. Fangying Chen
  6. Harry Li
  7. Claire Lin
  8. Elizabeth A. Komives
  9. Omar S. Akbari

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Abstract

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  1. Version published to 10.1016/j.jmb.2025.169566
  3. Arcadia Science

    Here we demonstrate that the Cas7-11 proteins are modular and can be engineered into compact, efficient, and highly specific Cas7-S effectors for RNA-targeting applications.

    Did you explore other offtarget effects?

  4. Arcadia Science

    We also found Cas7-1 contains a conserved catalytic residue that aligns to all known Cas7.3 domains, as well as zinc-finger motifs unique to type III-E effectors (4) (Figure 1B-E). Lastly, the Cas1 domain aligns to a Reverse Transcriptase-Cas1 (RT-Cas1) fusion protein commonly associated with type III systems of all characterized subtypes (III-A, III-B, III-C, and III-D) (Figure 1F, File S1).

    Excellent paper. Why do you think Cas7-1 is missing the highly conserved zinc finger residues in the 499-518 region (Fig 1C)? It seems that the loss of these residues also evolved in the CsbCas7-11 system as well.

  5. Version published to 10.1101/2024.02.23.581838 on bioRxiv