Stimulated Prorocentrum donghaiense cell growth by in-situ mariculture dissolved organic matter
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Abstract
Mixotrophic dinoflagellates frequently cause harmful algal blooms (HAB) in eutrophic mariculture waters that contain diverse excreted dissolved organic matter (DOM). The phagotrophy and the utilization of single organic small molecules have been extensively investigated in the bloom-forming mixotrophic dinoflagellates. However, their ability to utilize the in-situ DOM via absorbtrophy still remains unexplored. Here we examined the growth promotion effect of the in-situ mariculture DOM on Prorocentrum donghaiense , a representative HAB-forming species in coastal waters. Our results showed that the cell growth and photosynthesis of P. donghaiense were significantly promoted under in-situ DOM culture conditions. Additionally, parallel cultures were set up to disclose the potential role of the bacterioplankton in the free-living community (helper), where they aid in the remineralization of the in-situ DOM, and the phycosphere community (competitor), where they compete against the algal host to acquire nutrients from the in-situ DOM. Meanwhile, we determined the cellular stoichiometry under different culture conditions, showing that mariculture DOM can shape cellular stoichiometry significantly. Elevated cellular N (84.96%) and P (48.3%) were observed in spring DOM groups compared with the control groups. For the first time, this study quantifies the efficient utilization of the in-situ DOM via absorbtrophy, indicating the vital role in the outbreak and maintenance of HAB events.
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Besides that, autumn-DOM exhibited a higher promotion of cell growth (Fig. 2c, d). Considering the projected warming scenario in the future, we propose a hypothesis that the outburst of HAB event in early autumn is likely to happen in ECS coastal waters, which may contribute to higher frequency of HABs shown in the model simulation (Xiao et al., 2018; Xiao et al., 2019; Zhou et al., 2022).
Thank you for sharing this important work. It seems that there are innumerable variables involved in the frequency of HABs and climate change will undoubtedly impact each of these. It seems these experiments were run at ambient temperature in the lab, correct? I assume the temperature in the estuary is wildly different in the spring & autumn (and throughout the day) which will impact the nutrients available in the DOM sample drastically. The NCMA …
Besides that, autumn-DOM exhibited a higher promotion of cell growth (Fig. 2c, d). Considering the projected warming scenario in the future, we propose a hypothesis that the outburst of HAB event in early autumn is likely to happen in ECS coastal waters, which may contribute to higher frequency of HABs shown in the model simulation (Xiao et al., 2018; Xiao et al., 2019; Zhou et al., 2022).
Thank you for sharing this important work. It seems that there are innumerable variables involved in the frequency of HABs and climate change will undoubtedly impact each of these. It seems these experiments were run at ambient temperature in the lab, correct? I assume the temperature in the estuary is wildly different in the spring & autumn (and throughout the day) which will impact the nutrients available in the DOM sample drastically. The NCMA collection center indicates this species can be maintained from 14-22 C. Have you tried maintaining the strains in the DOM while mimicking the temperatures of the seasonal samples?
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In this study, mariculture DOM served as an effective nutrient source promoting the growth of bloom forming species P. donghaiense, the growth rate was elevated 87.2% in comparison with the control group supplied with inorganic nutrients (Fig. 2)
I didn't see this in the text, but are HAB in the Changjiang Esturay more common in the spring or autumn?
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Fig. 5
Sorry, the text in this figure (and to a lesser extent fig 6) is very hard to read. The text and image is very pixelated.
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4.1 Promoted cell growth by mariculture DOM
Just out of curiousity, how does the growth in DOM compare to growth in standard culture mediums like L1 or F/2?
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