The maintenance of centriole appendages and motile cilia basal body anchoring relies on TBCCD1
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Abstract
Centrosomes are organelles consisting of two structurally and functionally distinct centrioles, with the mother centriole having complex distal (DA) and subdistal appendages (SDA). Despite their importance, how appendages are assembled and maintained remains unclear. This study investigated human TBCCD1, a centrosomal protein essential for centrosome positioning, to uncover its localization and role at centrioles. We found that TBCCD1 localizes at both proximal and distal regions of the two centrioles, forming a complex structure spanning from SDA to DA and extending inside and outside the centriole lumen. TBCCD1 depletion caused centrosome mispositioning, which was partially rescued by taxol, and the loss of microtubules (MTs) anchored to centrosomes. TBCCD1 depletion also reduced levels of SDA proteins involved in MT anchoring such as Centriolin/CEP110, Ninein, and CEP170. Additionally, TBCCD1 was essential for the correct positioning of motile cilia basal bodies and associated structures in Paramecium . This study reveals that TBCCD1 is an evolutionarily conserved protein essential for centriole and basal body localization and appendage assembly and maintenance. A BioID screening also linked TBCCD1 to ciliopathy-associated protein networks.
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Our results strongly support that TBCCD1 loss of function compromised the symmetry of the MT centrosomal aster, causing both a dispersion of satellites in the cytoplasm, and a concomitant decrease in their pericentrosomal localization, supporting the previous evidence that the number of MTs anchored to the centrosome is lower in these cells.
I think this conclusion is the most difficult to support from the data shown in figure 1 a-c and B. The images seem of insufficient resolution in a-c to claim unanchored microtubules along with subtle differences in satellite distribution based on mean distance or percentage within a window.
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we show that these aggregates were in a distinct plane to that of the two centrioles that are separated
Seems like the radial pattern of control MTs may just be exacerbated due to physical/constraint proximity to the nucleus rather than a particular directional growth. It would also be be helpful to see cell morphology via brightfield/other in these images to see what the other membrane or cellular features might contribute to this polarization.
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