Cell specification and functional interactions in the pig blastocyst inferred from single-cell transcriptomics and uterine fluids proteomics
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Abstract
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conserved role of IL1B genes between pig and human/monkey
Since you didn't include mice here, am I correct to assume that this is something that is NOT conserved in mice. And therefore an advantage to using pig as a model for human biology?
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Figure 1A
This was super helpful. Thank you!
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earch to improve the derivation, stability and survival of porcine ESCs.
Maybe this is leftover from a previous version?
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Proteomic datasets were also generated from the uterine fluids of the sows used for embryo production
As an outsider to this field, it would be helpful for me to better understand the rationale for including uterine fluids. It seems important, and I want to make sure I understand why!
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The early stage shows a protein intensity profile with functions associated with cell metabolism, such as those involved in glycolysis GAPDH, ENO1, AKR1A1, PKM, IDH1 (Figure 6B) [53]–[57] pyruvate mechanism LDHA/B (Figure 6B) [58] and proteins with pleiotropic functions such as proteins of 14-3-3 and YWHAQ/Z/E families, recently identified as key players during the maternal-to-zygotic transition in pigs (Figure 6B) [59].
Would be curious to know your interpretation of this result. Do you think these proteins are being secreted by the uterine lining or the embryo itself? i.e. in which direction is communication happening?
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whose transcriptional profiles are very different from the later lineages
Curious if these transcriptional profiles provided any insights for you on other proxies you could measure in the future that may help you differentiate between cell types and stages? i.e. key metabolites/hormones that could be assayed for instead of sequencing?
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