The importance of cellulolytic and xylanolytic activities of Bacillus subtilis on dairy cow feed digestibility
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Abstract
A large body of literature has accumulated on the beneficial impact of the administration of Bacilli to dairy cows, particularly on dry matter intake, lactation performances and milk composition. In this work we sought to establish whether the ability of B. subtilis to secrete cellulase and xylanase enzymes could be involved in the positive effect exerted by these bacteria.
Several feed ingredients were treated with two B. subtilis strains only differing for the amount of secreted cellulosolytic and xylanolytic enzymes, and feed quality was assessed. We found that in vitro fibre digestibility correlated with the activity of those enzymes. Our results revealed that B. subtilis cellulases and xylanases can effectively improve forage quality, providing a scientific rationale on the use of Bacilli as forage supplements to improve animal productivity. Moreover, a particular care was taken in designing a sustainable and economically viable Bacillus -based additive preparation process.
Article activity feed
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Thank you for the great publication! It's really important to understand how to improve feed digestibility and you have listed several great reasons. Based on this article and your previous work, it seems that it's possible to improve the secretion levels of xylanase and cellulase enzymes. I'm an enzymologist by training so I was wondering how difficult would it be to improve the actual activity of such hydrolytic enzymes? It could be even more helpful to increase not only the enzyme amount secreted by B. subtilis but also the degradative activity of each enzyme molecule. Could these enzymes be expressed in E. coli to perform a high-throughput mutagenesis screen? Or perhaps such a screen could also be conducted in B. subtilis? Thank you so much for your time!
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Thank you for the great publication! It's really important to understand how to improve feed digestibility and you have listed several great reasons. Based on this article and your previous work, it seems that it's possible to improve the secretion levels of xylanase and cellulase enzymes. I'm an enzymologist by training so I was wondering how difficult would it be to improve the actual activity of such hydrolytic enzymes? It could be even more helpful to increase not only the enzyme amount secreted by B. subtilis but also the degradative activity of each enzyme molecule. Could these enzymes be expressed in E. coli to perform a high-throughput mutagenesis screen? Or perhaps such a screen could also be conducted in B. subtilis? Thank you so much for your time!
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