Optimization of extraction, Structural characterization of immunomodulatory acidic polysaccharide from Euphorbia caducifolia
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Abstract
Heating confluence method in combination with response surface methodology (RSM) was performed to optimize polysaccharides extraction from Euphorbia caducifolia Haines. The Box-Behnken Design was used to optimize the extraction conditions with three parameters such as temperature (A), Solid to liquid ratio (B), and time (C). The percentage yields obtained were fitted to a 2 nd order polynomial equation using multiple regression analysis. From the regression equation, the optimal polysaccharides extraction parameters for 2.3% of yield are Temperature-120°C, Solid to liquid ratio-75 g/L, and Time-2 h. Further, crude polysaccharide was purified and disclosed GlaUA, Rha, Glc, Gal, Xyl and Ara (1:2.5:4:3.6:3.7:3.3) ratio of poysaccharide. Thermogram of the EC polysaccharide showed typical natural polysaccharide behavior, and hydrophilic nature of polysaccharide attributed to endothermic water loss. Furthermore, purified polysaccharides exhibited strong in vitro free radical scavenging activities, reducing ability, and immunomodulatory activity by the effective production of pro-inflammatory cytokines with RAW 264.7 cells.
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This Zenodo record is a permanently preserved version of a PREreview. You can view the complete PREreview at https://prereview.org/reviews/10171568.
The provided comment pertains to a preprint article discussing Euphorbia caducifolia polysaccharides, a cactus native to India and Pakistan, with a focus on structure elucidation, extraction, antioxidant and immunomodulation properties, as well as emulsifying potential. The work involved extensive efforts in purifying and characterizing these polysaccharides through various methodologies.
Major issues
We suggest a more comprehensive introduction to enhance the quality and impact of this preprint. Such an introduction could contextualize the different types of polysaccharides found in nature, shedding light on the significance of the study. For instance, specifying that the target …
This Zenodo record is a permanently preserved version of a PREreview. You can view the complete PREreview at https://prereview.org/reviews/10171568.
The provided comment pertains to a preprint article discussing Euphorbia caducifolia polysaccharides, a cactus native to India and Pakistan, with a focus on structure elucidation, extraction, antioxidant and immunomodulation properties, as well as emulsifying potential. The work involved extensive efforts in purifying and characterizing these polysaccharides through various methodologies.
Major issues
We suggest a more comprehensive introduction to enhance the quality and impact of this preprint. Such an introduction could contextualize the different types of polysaccharides found in nature, shedding light on the significance of the study. For instance, specifying that the target polysaccharide belongs to the pectin class and delving into the specific characteristics of this type of polysaccharide would facilitate understanding and connect better with the intended biological and technological applications.
Regarding methodology, including more descriptive elements, particularly concerning experiment replication, would be beneficial. Additionally, it is crucial to provide references within affirmative sentences and incorporate up-to-date articles to provide context for the research. Specific clarification is needed regarding the optimization experiments, especially concerning the use of a temperature of 120°C, which exceeds the boiling point of water. It should be explained whether extractions were conducted in a pressure reactor to maintain water in a liquid state at this temperature. Further elaboration is necessary.
Linkage analysis data can be intricate to analyze, and it appears that the authors faced challenges interpreting it. Revisiting this section and rewriting it after a thorough data analysis is advisable. Emphasizing the value of purifying and determining the possible structure of this polysaccharide, even in a preliminary manner, is paramount. Therefore, a greater focus on carbohydrate chemistry, along with a deeper data analysis and discussion, would significantly enhance the article's scientific merit.
Minor issues
Additionally, improving the quality of graphics and providing a more extensive discussion of the results would enhance the visual appeal and comprehensibility of the findings.
Competing interests
The author declares that they have no competing interests.
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