An 8-cage imaging system for automated analyses of mouse behavior
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Abstract
The analysis of mouse behavior is used in biomedical research to study brain function in health and disease. Well-established rapid assays allow for high-throughput analyses of behavior but have several drawbacks, including measurements of daytime behaviors in a nocturnal animal, effects of animal handling, and the lack of an acclimation period in the testing apparatus. We developed a novel 8-cage imaging system, with animated visual stimuli, for automated analyses of mouse behavior in 22-hour overnight recordings. Software for image analysis was developed in two open-source programs, ImageJ and DeepLabCut. The imaging system was used to measure multiple behaviors, including acclimation to the novel cage environment, day and nighttime activity, stretch-attend postures, location in various cage areas, and habituation to animated visual stimuli. These behaviors were summarized in behavioral profiles, which may be used in further studies to examine treatments for neural disorders.
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The two camera feeds
Maybe I missed it elsewhere in the text, but could you provide detail about the cameras and capture settings?
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Various behaviors were summarized in a behavioral profile
Did you validate these behavioral data with manual analysis of the videos?
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(1) nose, (2) right ear, (3) left ear, (4) head, (5) neck, (6) center, (7) hip, (8) tail base, (9) right front paw, (10) left front paw, (11) right rear paw, (12) left rear paw
Wow, great! Did DLC reliably call all of these positions in most frames?
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