Endozoicomonas -chlamydiae interactions in cell-associated microbial aggregates of the coral Pocillopora acuta

  1. Justin Maire
  2. Kshitij Tandon
  3. Astrid Collingro
  4. Allison van de Meene
  5. Katarina Damjanovic
  6. Cecilie Ravn Gøtze
  7. Sophie Stephenson
  8. Gayle K. Philip
  9. Matthias Horn
  10. Neal E. Cantin
  11. Linda L. Blackall
  12. Madeleine J. H. van Oppen

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Abstract

Corals are associated with a variety of bacteria, which occur in the surface mucus layer, gastrovascular cavity, skeleton, and tissues. Some tissue-associated bacteria form clusters, termed cell-associated microbial aggregates (CAMAs), which are poorly studied. Here, we provide a comprehensive characterization of CAMAs in the coral Pocillopora acuta . Combining imaging techniques, laser capture microdissection, and amplicon and metagenome sequencing we show that CAMAs: (i) are located in the tentacle tips and may be intracellular; (ii) contain Endozoicomonas, Kistimonas (both Gammaproteobacteria), and Simkania (Chlamydiota) bacteria; (iii) Endozoicomonas may provide vitamins to its host and use secretion systems and/or pili for colonization and aggregation; (iv) Endozoicomonas and Simkania occur in distinct, but adjacent, CAMAs; (v) Simkania may rely on acetate and heme provided by neighboring Endozoicomonas . Our study provides detailed insight into coral endosymbionts, which will guide the assessment of their suitability for probiotic approaches to mitigate coral bleaching.

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  1. Arcadia Science

    Data availability: Raw data are available under NCBI BioProject IDs PRJNA891910 (Adult1122CAMAs, MiSeq and NovaSeq raw data, MAGs), PRJNA891898 (Larva CAMAs, MiSeq raw1123data), PRJNA891892 (Whole larva microbiome, MiSeq raw data).

    These are such exciting data sets! I tried searching for them on the SRA and the ENA the accessions could not be found. Would you be willing make your data public now that you have released your preprint?

  2. Arcadia Science

    Data availability: Raw data are available under NCBI BioProject IDs PRJNA891910 (Adult1122CAMAs, MiSeq and NovaSeq raw data, MAGs), PRJNA891898 (Larva CAMAs, MiSeq raw1123data), PRJNA891892 (Whole larva microbiome, MiSeq raw data).

    These are such exciting data sets! I tried searching for them on the SRA and the ENA the accessions could not be found. Would you be willing make your data public now that you have released your preprint?

  3. Version published to 10.1101/2022.11.28.517745v1 on bioRxiv
  4. Version published to 10.1101/2022.11.28.517745v2 on bioRxiv