Two-Photon Targeted, Quad Whole-Cell Patch-Clamping Robot
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Abstract
We present an automated quad-channel patch-clamp technology platform for ex vivo brain slice electrophysiology, capable of both blind and two-photon targeted robotically automated patching. The robot scales up the patch-clamp singlecell recording technique to four simultaneous channels, with seal success rates for two-photon targeted and blind modes of 54% and 68% respectively. In 50% of targeted trials (where specific cells were required), at least 2 simultaneous recordings were obtained. For blind mode, most trials yielded dual or triple recordings. This robot, a milestone on the path to a true in vivo robotic multi-patching technology platform, will allow numerous studies into the function and connectivity patterns of both primary and secondary cell types.
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It is the centre of mass that is used as the x/y targetcoordinates for the pipettes, so as to patch the centre of thecell, while the z-coordinate comes from the plane at whichthe cell is most in focus.
Do you have situations when the cell moves out of the way and you have to take the pipette out and try again?
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After each trial, pipettes are automatically cleaned in anenzymatic solution and then returned to their initial positions,allowing for reuse,
was wondering how often you guys encounter clogging from debris in the pipette after cleaning? we saw this happen a lot especially if the pipette was close to vertical position
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