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  2. SciScore for 10.1101/2020.10.31.362848: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    NIH rigor criteria are not applicable to paper type.

    Table 2: Resources

    Antibodies
    SentencesResources
    Plates were washed and sequentially incubated with 1 μg/mL of CR3022 (Yuan et al., 2020) anti-S-protein antibody and HRP-conjugated goat anti-human IgG in PBS, 0.1% saponin, 0.1% BSA.
    anti-S-protein
    suggested: None
    anti-human IgG
    suggested: None
    Experimental Models: Cell Lines
    SentencesResources
    Vero E6 (ATCC), HEK293T (ATCC) and HEK293T cells stably overexpressing ACE2 were maintained at 37 °C in 5% CO2 in DMEM containing 10% (vol/vol) FBS.
    HEK293T
    suggested: None
    Infection assays with pseudotyped VLPs: HEK-293 cells stably over-expressing full-length human ACE2 protein were seeded in 96-well white polystyrene microplates (Corning, Cat. No. CLS3610) at 0.03 × 106 cells/well in DMEM (10% FBS and 1% penicillin-streptomycin) and were grown overnight at 37 °C with 5% CO2.
    HEK-293
    suggested: None
    Virus stocks were produced in Vero CCL81 cells (ATCC) and titrated by focus-forming assay on Vero E6 cells (Case et al., 2020).
    Vero CCL81
    suggested: None
    Vero E6
    suggested: None
    The Fabs were expressed from HEK293F cells.
    HEK293F
    suggested: RRID:CVCL_6642)
    Software and Algorithms
    SentencesResources
    The data were analyzed by GraphPad Prism Version 8.4.3 (GraphPad Software, LLC).
    GraphPad Prism
    suggested: (GraphPad Prism, RRID:SCR_002798)
    GraphPad
    suggested: (GraphPad Prism, RRID:SCR_002798)
    Data were processed using Prism software (GraphPad Prism 8.0).
    Prism
    suggested: (PRISM, RRID:SCR_005375)
    The atomic models were built using Coot (Emsley et al., 2010) and refined with Phenix.refine (Afonine et al., 2012).
    Coot
    suggested: (Coot, RRID:SCR_014222)
    The particle images corresponding to Fab-S-protein complexes in the different conformations were separated by performing heterogenous refinement in cryoSPARC v2.
    cryoSPARC
    suggested: (cryoSPARC, RRID:SCR_016501)
    The atomic models were validated using Molprobity (Williams et al., 2018) and the comprehensive validation (cryo-EM) tool in Phenix (Liebschner et al., 2019).
    Molprobity
    suggested: (MolProbity, RRID:SCR_014226)
    Phenix
    suggested: (Phenix, RRID:SCR_014224)

    Results from OddPub: Thank you for sharing your data.


    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: We found the following clinical trial numbers in your paper:

    IdentifierStatusTitle
    NCT04452318RecruitingCOVID-19 Study Assessing the Efficacy and Safety of Anti-Spi…
    NCT04497987RecruitingA Study of LY3819253 (LY-CoV555) and LY3832479 (LY-CoV016) i…


    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.


    Results from JetFighter: We did not find any issues relating to colormaps.


    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

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