Age-related decline in behavioral discrimination of amplitude modulation frequencies compared to envelope-following responses
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Abstract
The ability to discriminate modulation frequencies is important for speech intelligibility because speech has amplitude and frequency modulations. Neurophysiological responses assessed by envelope following responses (EFRs) significantly decline at faster amplitude modulation frequencies (AMF) in older subjects. A typical assumption is that a decline in EFRs will necessarily result in corresponding perceptual deficits. To test this assumption, we investigated young and aged Fischer-344 rats’ behavioral AMF discrimination abilities and compared to their EFRs. A modified version of prepulse inhibition (PPI) of acoustic startle reflex (ASR) was used to obtain behavioral performance. A PPI trial contains pulses of sinusoidal AM (SAM) at 128 Hz presented sequentially, a SAM prepulse with different AMF and a startle-eliciting-stimulus. To account for hearing threshold shift or age-related synaptopathy, stimulus levels were presented at 10-dB lower or match to the aged peripheral neural activation (using auditory brainstem response wave I amplitude). When AMF differences and modulation depths were large, young and aged animals’ behavioral performances were comparable. Aged animals’ AMF discrimination abilities declined as the AMF difference or the modulation depth reduced, even compared to the young with peripheral matching. Young animals showed smaller relative decreases in EFRs with reduced modulation depths. The correlation of EFRs and AM perception was identified to be more consistent in young animals. The overall results revealed larger age-related deficits in behavioral perception compared to EFRs, suggesting additional factors that affect perception despite smaller degradation in neural responses. Hence, behavioral and physiological measurements are critical in unveiling a more complete picture on the auditory function.
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This Zenodo record is a permanently preserved version of a PREreview. You can view the complete PREreview at https://prereview.org/reviews/7621012.
Systems JC, OHSU - PREreview of "Age-related decline in behavioral discrimination of amplitude modulation frequencies compared to envelope-following responses"
This is a preprint journal club review of "Age-related decline in behavioral discrimination of amplitude modulation frequencies compared to envelope-following responses" by Jesyn Lay, Edward L. Bartlett. The preprint was posted on bioRxiv on Sep 28, 2017 (DOI: http://dx.doi.org/10.1101/193268).
Our group reviewed this paper on October 13, 2017 and used the PREreview quick participant worksheet.
Dear Authors,
Thank you for posting this manuscript as a preprint on bioRxiv! We reviewed this work at our Systems Neuroscience …
This Zenodo record is a permanently preserved version of a PREreview. You can view the complete PREreview at https://prereview.org/reviews/7621012.
Systems JC, OHSU - PREreview of "Age-related decline in behavioral discrimination of amplitude modulation frequencies compared to envelope-following responses"
This is a preprint journal club review of "Age-related decline in behavioral discrimination of amplitude modulation frequencies compared to envelope-following responses" by Jesyn Lay, Edward L. Bartlett. The preprint was posted on bioRxiv on Sep 28, 2017 (DOI: http://dx.doi.org/10.1101/193268).
Our group reviewed this paper on October 13, 2017 and used the PREreview quick participant worksheet.
Dear Authors,
Thank you for posting this manuscript as a preprint on bioRxiv! We reviewed this work at our Systems Neuroscience journal club at OHSU. Compiled comments from the attendants are below. To structure the feedback used the quick worksheet guidelines published on PREreview. We hope this feedback will be useful to improve the manuscript.
What is the main question the study attempts to answer?
This study attempts to establish a relation between significant decline of auditory neural responses measured by envelope following responses (EFRs) and perceptual deficits in detecting fast amplitude-modulated frequency sounds associated with age-related hearing loss in rats.
What is (are) the hypothesis(es)?
The authors hypothesized that the well know decline in EFR in older rats in response to faster amplitude-modulated frequencies (AMF) is accompanied by a deficit in AM perception of those sounds.
Why is this study relevant?
Human speech contains fast modulations in amplitude and frequency that are crucial for speech intelligibility. Age-related hearing loss in humans is associated with difficulty in understanding speech often as a consequence of poor temporal modulation coding. Understanding if the lower electrophysiological responses to fast AMF measured in aged rats are associated with behavioral deficits in the same animals is important to establish the link between humans and animal models of age-related hearing loss.
What techniques/analyses do the researchers adopt to test their hypothesis(es)?
Behavioral tests:
- Acoustic startle response (ASR) and inhibition of the startle reaction using a prepulse (prepulse inhibition, PPI). The magnitude of the PPI is proportional to the animal's sound detectability such as the highest the PPI response, the ...
- Pure tone detection behavior.
- AMF discrimination task
Electrophysiological measurements
- Auditory evoked potentials as auditory brainstem responses (ABRs) and envelop following responses (EFRs).
Write here any specific comment you might have about experimental approaches and methods used in the study.
Write here any general comments you might have about the research approach.
Write here any specific comment/note about figures in the paper (this could be related to the way data are displayed and your ability to understand the results just by looking at the figures).
It would be useful to include a diagram of the behavioral paradigms to help readers understand how the experiment is set up.
In Figure 1, to make it clear where you measure the startle response and to show the actual shape of the measured response you could use figure 2 waveform of the startle response instead of the square pulse. We would recommend combining these figures into one figure, perhaps with two panels.
Figure 3. It would be nice to show the psychometric functions as it looks like you would get a good one of the ASR magnitude but not for RMS ratio. This would show clearly that ASR magnitude measurements are a better way to measure behavioral thresholds (level at which there is no measurable change in startle response), key information to establish a link with human perceptual measurements. From now on, you could avoid showing the RMS ratio measurement in the main figures, and perhaps have them in the supplementary or report the statistics only in the text?
Table 1 is a bit confusing as it's hard to understand what the important part is. Could you for instance highlight relative to 75 the first level that has a significantly lower response?
In Figure 4 it would help to label that the change in AM frequency is related to the prepulse stimulus.
Figure 6 is very dense and hard to interpret. What are the matched waves? It's a bit confusing to understand what's going on because sometimes the response is even higher in the matched condition. Would it be useful to visualize the mean of the matched intensity.
Figure 7 is very interesting. Even after you equated the levels, you still got much less PPI in the older animals than in the younger. This would suggest that there is something happening at higher levels of auditory processing.
In Figure 8 for visualization purposes, it would be better if the solid solid line were the 100% modulation depth. Additionally, to make the figure more clear you could remove the 25% for young as you don't have that measurement in all conditions.
Figure 9 perhaps should be brought up in the preprint because if left at the end after the references it is not easily accessible to the reader. This figures attempts to compare behavioral results with electrophysiological measurements. The message of this figure is that the behavior of the animal changes more compared to the electorphy responses. However it is a bit hard to compare a ratio with a linear scale. Perhaps it would be easier to have one panel that shows the change with 100% PPI and another with the change in EFR and compare between groups rather than comparing between measures?
One panel with change in 100% PPI and another with change in EFR and then you compare between the groups rather than comparing between the measures? We did not understand what the baseline was. Did you try doing everything in a multiple linear regression? Does EFR predict PPI on individual level not for averages. PPI=a+b*EFR.
The relative change affects the behavior rather than the absolute repsonse? the 50% depth and 100% are similar in younger but performance looks quite different. It appears that the relative change in EFR does not predict the behavior. Is that correct?
Write here any additional comment you might have (this includes minor concerns such as typos and structure of the manuscript)
Introduction:
- I wonder if you need to go into such details to understand the circuit in order to motivate the study. I would focus on stating the problem, what's known about the problem, what is/are you hypothesis/es, and how you are going to test it/them. Conclude the intro with a short paragraph about your findings. Leave the description of the methods to the method session. P
- Perhaps it would be helpful to clarify the distinction between previous findings in human and animal models. It could be important to report any psychophysical evidence that supports how older humans have harder time understanding fast speech. Additionally, you might want to mention that the way that the startle response circuit is wired in humans is different from the rat.
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