Automated molecular detection of the vancomycin resistance genes vanA and vanB using the geneLEAD VIII platform
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Rapid and accurate detection of vancomycin-resistant enterococci (VREs) can aid in the early application of appropriate antimicrobial treatment and the implementation of infection prevention measures. The VIASURE real-time PCR assay (Certest Biotec) is a multiplex nucleic acid-based in vitro diagnostic test intended for the detection of vanA and vanB and geneLEAD Ⅷ (Precision System Science) is a customizable, fully automated molecular detection platform. We evaluated the performance of the VIASURE assay on the geneLEAD Ⅷ platform against 200 clinical enterococcal isolates consisting of 169 VRE and 31 vancomycin-susceptible enterococci collected in Japan, primarily in the Kinki region, and compared it to that of the reference multiplex PCR assay. The performance of the VIASURE assay for the detection of vanA and vanB was comparable to that of the reference multiplex PCR assay, with both a sensitivity and specificity of 100%. Compared with the reference PCR assay, the VIASURE assay reduced the turn-around time by approximately 3 h 5 min and the hands-on time by 2 h 45 min per four samples. Fully automated molecular detection of vanA and vanB using the VIASURE assay and geneLEAD VIII can enable fast and reliable testing while reducing labor and is a promising tool for nosocomial infection control.
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Thank you very much for submitting your manuscript to Access Microbiology. I have read through it and the content will potentially be of interest in the field of diagnostics. However, I would like to kindly ask you to do some modifications before progressing to peer review: - Please consider changing the article type to "Short communication", as your manuscript seems to be below 3,000 words. - It is a bit hard to follow and understand the content of the tables (particularly Table 3), as there are no cell borders. Please consider changing the table format to an alternative clearer version. Please provide a revised version of the manuscript introducing these changes within a month.
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