A databank for intracellular electrophysiological mapping of the adult somatosensory cortex

  1. Angelica da Silva Lantyer
  2. Niccolò Calcini
  3. Ate Bijlsma
  4. Koen Kole
  5. Melanie Emmelkamp
  6. Manon Peeters
  7. Wim J J Scheenen
  8. Fleur Zeldenrust
  9. Tansu Celikel

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Abstract

Background

Neurons in the supragranular layers of the somatosensory cortex integrate sensory (bottom-up) and cognitive/perceptual (top-down) information as they orchestrate communication across cortical columns. It has been inferred, based on intracellular recordings from juvenile animals, that supragranular neurons are electrically mature by the fourth postnatal week. However, the dynamics of the neuronal integration in adulthood is largely unknown. Electrophysiological characterization of the active properties of these neurons throughout adulthood will help to address the biophysical and computational principles of the neuronal integration.

Findings

Here, we provide a database of whole-cell intracellular recordings from 315 neurons located in the supragranular layers (L2/3) of the primary somatosensory cortex in adult mice (9–45 weeks old) from both sexes (females, N = 195; males, N = 120). Data include 361 somatic current-clamp (CC) and 476 voltage-clamp (VC) experiments, recorded using a step-and-hold protocol (CC, N = 257; VC, N = 46), frozen noise injections (CC, N = 104) and triangular voltage sweeps (VC, 10 (N = 132), 50 (N = 146) and 100 ms (N = 152)), from regular spiking (N = 169) and fast-spiking neurons (N = 66).

Conclusions

The data can be used to systematically study the properties of somatic integration and the principles of action potential generation across sexes and across electrically characterized neuronal classes in adulthood. Understanding the principles of the somatic transformation of postsynaptic potentials into action potentials will shed light onto the computational principles of intracellular information transfer in single neurons and information processing in neuronal networks, helping to recreate neuronal functions in artificial systems.

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  1. GigaScience

    Now published in GigaScience doi: 10.1093/gigascience/giy147

    Angelica da Silva Lantyer Department of Neurophysiology, Donders Institute for Brain, Cognition and Behaviour, Radboud University, Nijmegen - the NetherlandsFind this author on Google ScholarFind this author on PubMedSearch for this author on this siteNiccolò Calcini Department of Neurophysiology, Donders Institute for Brain, Cognition and Behaviour, Radboud University, Nijmegen - the NetherlandsFind this author on Google ScholarFind this author on PubMedSearch for this author on this siteAte Bijlsma Department of Neurophysiology, Donders Institute for Brain, Cognition and Behaviour, Radboud University, Nijmegen - the NetherlandsFind this author on Google ScholarFind this author on PubMedSearch for this author on this siteKoen Kole Department of Neurophysiology, Donders Institute for Brain, Cognition and Behaviour, Radboud University, Nijmegen - the NetherlandsFind this author on Google ScholarFind this author on PubMedSearch for this author on this siteMelanie Emmelkamp Department of Neurophysiology, Donders Institute for Brain, Cognition and Behaviour, Radboud University, Nijmegen - the NetherlandsFind this author on Google ScholarFind this author on PubMedSearch for this author on this siteManon Peeters Department of Neurophysiology, Donders Institute for Brain, Cognition and Behaviour, Radboud University, Nijmegen - the NetherlandsFind this author on Google ScholarFind this author on PubMedSearch for this author on this siteWim J. J. Scheenen Department of Neurophysiology, Donders Institute for Brain, Cognition and Behaviour, Radboud University, Nijmegen - the NetherlandsFind this author on Google ScholarFind this author on PubMedSearch for this author on this siteFleur Zeldenrust Department of Neurophysiology, Donders Institute for Brain, Cognition and Behaviour, Radboud University, Nijmegen - the NetherlandsFind this author on Google ScholarFind this author on PubMedSearch for this author on this siteTansu Celikel Department of Neurophysiology, Donders Institute for Brain, Cognition and Behaviour, Radboud University, Nijmegen - the NetherlandsFind this author on Google ScholarFind this author on PubMedSearch for this author on this site

    A version of this preprint has been published in the Open Access journal GigaScience (see paper https://doi.org/10.1093/gigascience/giy147 ), where the paper and peer reviews are published openly under a CC-BY 4.0 license.

    These peer reviews were as follows:

    Reviewer 1: http://dx.doi.org/10.5524/REVIEW.101446 Reviewer 2: http://dx.doi.org/10.5524/REVIEW.101447 Reviewer 3: http://dx.doi.org/10.5524/REVIEW.101448

  2. Version published to 10.1093/gigascience/giy147
  3. Version published to 10.1101/472043v1 on bioRxiv