Label-free in-line characterization of immune cell culture using quantitative phase imaging
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Abstract
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From these images, we obtain a pixel-wise dynamic frequency response given by the absolute value of the Fourier transform of the temporal phase signal,
for each spatial pixel in the image.Just to clarify: are these the absolute pixels in the entire imaging field? or the pixels of individual segmented cells? if segmented cells, was there any registration of the images? Are the cells moving or do you have evidence that some of these changes in the phasor analysis don't result from jitter in the positions of the cells?
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Here, we take a stack of 600 sequential qOBM images taken at 8 Hz (this frame rate was selected to capture metabolic activity within the cell)
Is the expectation here that metabolic changes will cause changes in the refactive index on a time scale of greater than 8 Hz? Is that a realistic time frame for metabolic changes? or are these other structural changes in response to activation?
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