APEX: Automated Protein EXpression in Escherichia coli
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However, when the objective is to express or screen proteins in high-throughput, the requirement for single colonies can be relaxed, and non isogenic cultures can be used.
It was great to read how you were able achieve this on the OT2. I'm wondering, if cultures can be non isogenic, wouldn't it be more straightforward to transfer transformed cultures to selective liquid media plates instead of dealing with the complexities of calibrating for agar plates? I'd love to hear your thoughts, thanks!
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