APEX: Automated Protein EXpression in Escherichia coli

  1. Martyna Kasprzyk
  2. Michael A. Herrera
  3. Giovanni Stracquadanio

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  1. Mentioned by @profstracquadanio.bsky.social on Bluesky

    Excited to share our new publication in ACS Synthetic Biology! We introduce #APEX - an open-source, automated pipeline for recombinant protein production in E. coli, developed by our super talented Martyna Kasprzyk! Check it out: pubs.acs.org/doi/10.1021/...

  2. Mentioned by @oborkowski.bsky.social on Bluesky

    APEX: Automated Protein EXpression in Escherichia coli doi.org/10.1021/acss...

  3. Version published to 10.1021/acssynbio.5c00189
  4. Arcadia Science

    However, when the objective is to express or screen proteins in high-throughput, the requirement for single colonies can be relaxed, and non isogenic cultures can be used.

    It was great to read how you were able achieve this on the OT2. I'm wondering, if cultures can be non isogenic, wouldn't it be more straightforward to transfer transformed cultures to selective liquid media plates instead of dealing with the complexities of calibrating for agar plates? I'd love to hear your thoughts, thanks!

  5. Version published to 10.1101/2024.08.13.607171v2 on bioRxiv
  6. Mentioned by @biorxiv-synthbio.bsky.social on Bluesky

    APEX: Automated Protein EXpression in Escherichia coli https://www.biorxiv.org/content/10.1101/2024.08.13.607171v1

  7. Mentioned by @biorxivpreprint.bsky.social on Bluesky

    APEX: Automated Protein EXpression in Escherichia coli https://www.biorxiv.org/content/10.1101/2024.08.13.607171v1

  8. Version published to 10.1101/2024.08.13.607171v1 on bioRxiv