A Miniaturized, High-Throughput Aqueous Solvent-Centric Method for Protein Solubility Screening
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Abstract
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extraction buffers are dispensed
what volume of extraction buffer do you use here? Information on the ratio of 'dried pellet material' to extraction buffer would be helpful here.
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dot-blot signal
What are the advantages of the dot blot over a conventional sds-page analysis? SDS-PAGE likely takes the same amount of time as a dot blot, but potentially gives you more information about each sample.
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provided in
This might just be an artifact of biorxiv, but I don't see a table description anywhere. What were the adverse reactions for the SDS-PAGE? Did the gel just not run straight or stain evenly? Also, what are the units for purification yield?
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suffice
If you are growing the cells in a single flask, at which step do you split up the pellet into a 96-well plate?
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d than optimized IPTG
It makes sense that you use the auto-induction for more high throughput purposes. After finding an optimal condition and scaling up, would you recommend switching to IPTG? Would you expect any changes?
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