A CPF-like phosphatase module links transcription termination to chromatin silencing
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The work here identifies the importance of the CPSF phosphatase module, a regulator of RNA Pol II,307in FLC regulation
This is a beautiful study describing the regulation of the FLC locus. Do you believe APRF1, TOPP4, and LD are specific regulators of only FLC? Or do they play a more general role in regulating repression/activation at many loci? If so, do you have any genome-wide information on how APRF1, TOPP4, and LD mutants effect other genes, particularly those involved in sensing environment cues, like you've examined with flowering? And, if their role is not restricted to regulating FLC, what other phenotypes do you observe with these mutants--do the phenotypes give you insights into other processes they may regulate?
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Taken together, the robust202immunoprecipitation of LD and TOPP4 by APRF1, the structural parallels between Ref2-PNUTS-LD203and the similar roles in co-transcriptional processing lead us to propose that LD, APRF1, and TOPP4204may form a functional CPSF phosphatase module in plants
It will be exciting to see if these putative subunits possess phosphatase activity, in vitro, and further dissect how this phosphatase activity might be regulated.
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Thus, APRF1 has a role in establishing a silent152chromatin status at FLC (Figure 1H, I)
The evidence that APRF1 is regulating flc expression is strong; however, given APRF1's likely role in regulating a wide range of genes, do you have evidence that APRF1 is directly associating with the flc locus to regulate expression and that the regulation is not indirect?
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