A conserved mechanism for the retrieval of polyubiquitinated proteins from cilia

  1. Sven M. Lange
  2. Jeremy A. Bennett
  3. Robyn J. Eisert
  4. Alan Brown

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Abstract

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  1. Version published to 10.1016/j.cell.2025.07.043
  2. Arcadia Science

    CFAP36 has broad ubiquitin-dependent specificity for membrane-associated proteins

    Do you see excess membrane budding/blebbing/ectocytosis in CFAP ubiquitin-binding mutants that can't retrieve membrane-associated cargo like in BBSome mutants?

  3. Arcadia Science

    TIRF microscopy analyses of these three strains revealed that while the mutations did not prevent CFAP36 from entering flagella, they inhibited its movement within the flagellum.

    Curious if you're actually in TIRF with this much cell body fluorescence and if you have WT CFAP36 data from this same experiment?

  4. Arcadia Science

    We discounted USP5 as an IFT adaptor because of its well-characterized function as a deubiquitinase

    Since deubiquitinases can be regulated, can function non-catalytically, and have specificity for ubiquitinated substrates, perhaps USP5 could still be a viable candidate

  5. Version published to 10.1101/2025.04.24.650332 on bioRxiv