Evaluated articles

eLife Assessment

This important study investigates how AD(H)D affects attention using neural and physiological measures in a Virtual Reality (VR) environment. Solid evidence is provided that individuals diagnosed with AD(H)D differ from control participants in both the encoding of the target sound and the encoding of acoustic interference. The VR paradigm here can potentially bridge lab experiments and real-life experiments. However, the reviewers identified a few potential technical issues that will need to be verified and discussed.

eLife Assessment

This study provides abundant valuable scRNA-Seq data that profiles fibroblasts involved in myocardium and coronary vasculature development. However, the evidence supporting the authors' claims is currently incomplete. The inclusion of additional citations, more in-depth discussions, and further analyses or experiments to validate the scRNA-Seq data would have significantly strengthened the study. Nonetheless, the scRNA-Seq expression data will be a resource that is of value to researchers in the field.

eLife Assessment

This important study aimed to identify how chronic heat exposure affects subsequent behavior and brain function. This work positively expands the field of thermoregulation. The data were collected using a myriad of next-generation approaches, including extensive behavior testing, thermal monitoring, electrophysiology, circuit mapping, and manipulations. As a result the strength of evidence is mostly solid, however a few weaknesses drove the some of the conclusions to be incompletely supported. These largely circle around the question of how unique these effects are to thermal stress (as opposed to other forms of stress), a lack of statistical analyses and rigor in some of the experiments and figures, and the specificity of the POA-pPVT pathway compared to other inputs to the PVT in the control of observed effects.

eLife Assessment

This valuable manuscript reports a large-scale, data-driven, biophysically detailed model of the non-barrel primary somatosensory cortex and generates numerous predictions that can further our understanding of how the multiscale organization of the cortex shapes neural activity. While the approach is solid, many of the findings are obtained using a much smaller portion of the model, which, together with the broad scope of the work, makes the narrative somewhat confusing and the strength of findings not entirely clear.

eLife Assessment

This useful study presents a biologically realistic, large-scale cortical model of the rat's non-barrel somatosensory cortex, investigating synaptic plasticity of excitatory connections under varying patterns of external activations and characterizing relations between network architecture and plasticity outcomes. While the model demonstrates several interesting phenomena, the results are less explanatory of causal relationships and more observational in nature; hence the evidence supporting the main conclusions remains incomplete.

eLife Assessment

The study is valuable to the field, introducing a new model to test BM-periosteal stem cell function in vivo. The authors' findings suggested that periosteal stem cells are linked to hematopoietic regeneration. More comparisons with the conventional model and direct examination of periosteal stem cell factors in hematopoietic regeneration are missing. The observations are solid, however, the limitations in their experimental model made the overall impact incomplete; there is potential for improvements to be made in this area.

eLife Assessment

This study presents important advances in the discovery and assessment of microcins that improve our understanding of their prevalence and roles. The bioinformatics analysis, expression, and antimicrobial assays are solid, although the diverging evaluations also indicated the need for additional support regarding the sequence analysis and validation to fully back some of the claims and conclusions. This study will appeal to researchers working on the discovery and analysis of novel peptide natural products.

eLife Assessment

This important study explores the interplay between gene dosage and gene mutations in the evolution of antibiotic resistance. The authors provide solid evidence to connect proteostasis with gene duplication during experimental evolution in a model system. If the experiments are found to be rigorous and reproducible, then this paper will be of high interest to other researchers studying antibiotic resistance, proteostasis, and bacterial evolution.

eLife Assessment

This study presents an important finding that ant nest structure and digging behavior depend on ant age demographics for a ground-dwelling ant species (Camponotus fellah). By asking whether ants employ age-polyethism in excavation, the authors address a long-standing question about how individuals in collectives determine the overall state of the task they must perform, and their results may prove to be a key consideration for interpreting results from other studies in the field of social insect behavior. While the experimental evidence that the age of the ants and the group composition affect the digging of tunnels is solid, some of the analyses and modeling are seem superfluous, as they do not further support the results or contribute to a deeper understanding of the system.

eLife Assessment

This useful study sheds light on the species-specific nature of sperm-oocyte interactions by examining sperm binding and penetration of the zona pellucida across various mammalian species. While the evidence remains incomplete, the authors propose that two distinct mechanisms drive mammalian sperm-oocyte recognition and penetration: a specific, zona pellucida (ZP)-mediated mechanism, and a non-specific, oviductal glycoprotein 1 (OVGP1)-mediated mechanism. Upon revision, this study would offer insights to reproductive biologists, potentially improving porcine in vitro fertilization (IVF) - which is particularly susceptible to polyspermy - and enhancing sperm selection processes in human IVF, ultimately leading to better outcomes in assisted reproduction techniques.

eLife Assessment

This is an important study examining the role of conserved PCH-2 protein at different stages of C. elegans meiosis. The authors use elegant molecular genetic approaches to provide convincing evidence to support their claims. The work will be of interest to scientists studying meiosis, DNA recombination, and chromosome segregation.

eLife Assessment

A regression discontinuity analysis finds essentially no effect of 1 additional year of secondary education on brain structure in adulthood. This is a valuable finding that adds to the literature on the impact of education on brain health. The evidence presented is solid, with strengths including methodological novelty as well as principled study design; the impact is, however, limited as the manipulated variable only relates to a single additional year of education (remaining in education to 15 vs 16 years of age). The interpretation is further missing discussion of the healthy volunteer bias of the UK Biobank sample, amplified in the imaging extension.

eLife Assessment

In this important study, the authors use zebrafish to examine protein absorption in the gut. Using a combination of imaging and single-cell RNA-seq, they characterize a population of lysosome-rich enterocytes that are essential for protein uptake. They find that the microbiome impacts the ability of these cells to uptake protein. The RNA-seq provides a rich dataset for future functional experiments, which makes a convincing case for the importance of these cells.

eLife Assessment

This useful study uses an intranasal mouse infection model with Streptococcus suis, a gram-positive bacterial pathogen that causes severe losses in pigs around the world. The manuscript provides insights that the capsular polysaccharide, one of the virulence factors of this pathogen, contributes to tissue dissemination and neurotropism in the host. However, the evidence is currently incomplete, and further experiments and careful interpretation of the current results and methods used are necessary to support the conclusions of the manuscript.

eLife Assessment

Fallah et al carefully dissect projections from substantia nigra pars reticulata (SNr) and the globus pallidus externa (GPe) – two key basal ganglia nuclei – to the pedunculopontine nucleus (PPN), a brainstem nucleus that has a central role in motor control. They consider inputs from these two areas onto 3 types of downstream PPN neurons – GABAergic, glutamatergic, and cholinergic neurons – and carefully map connectivity along the rostrocaudal axis of the PPN. Overall, this valuable study provided convincing data on PPN connectivity with two key input structures that will provide a basis for further understanding PPN function.

eLife Assessment

This important work investigates the mechanism that underlies the switch between feeding and mating behaviors in the oriental fruit fly, Bactrocera dorsalis. Using a variety of approaches, the authors show that this switch is mediated by the neuropeptide, sulfakinin, acting peripherally through the sulfakinin receptor 1 to regulate the expression of antennal odorant receptors. The evidence is solid in support of the hypothesis that sulfakinin signaling mediates changes in the periphery, although additional experimental details would strengthen these claims.

eLife Assessment

This valuable study uses robust time-dependent microscopy assays to show that during HIV-1 infection, the viral accessory protein Vif causes cell cycle arrest during metaphase and not G2/M as previously thought. The conclusions are convincing in the context of the immortalized cellular models used, and they serve as a starting point to determine whether Vif-dependent regulation of the cell cycle modulates HIV-1 replication and pathogenesis in more physiologically relevant primary cells or in vivo.

eLife Assessment

The manuscript provides important new insights into the mechanisms of statistical learning in early human development, showing that statistical learning in neonates occurs robustly and is not limited to linguistic features but occurs across different domains. The evidence is convincing, although an additional experimental manipulation with conflicting linguistic and non-linguistic information as well as further discussion about the linguistic vs non-linguistic nature of the stimulus materials would have strengthened the manuscript. The findings are highly relevant for researchers working in several domains, including developmental cognitive neuroscience, developmental psychology, linguistics, and speech pathology.

eLife Assessment

The study is important - not only for its comprehensive transcriptomic analysis of the developmental trajectory of syncytiotrophoblasts (STBs), but also for its comparative evaluation of primary human placental tissues and two human trophoblast organoid models. The study highlights the utility of these organoid models in advancing research on human STB biology. The conclusions of this work are supported by compelling analyses and experimental evidence.