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  1. Human airway cells prevent SARS-CoV-2 multibasic cleavage site cell culture adaptation

    This article has 11 authors:
    1. Mart M Lamers
    2. Anna Z Mykytyn
    3. Tim I Breugem
    4. Yiquan Wang
    5. Douglas C Wu
    6. Samra Riesebosch
    7. Petra B van den Doel
    8. Debby Schipper
    9. Theo Bestebroer
    10. Nicholas C Wu
    11. Bart L Haagmans
    This article has been curated by 1 group:
    • Curated by eLife

      Evaluation Summary:

      This manuscript follows up on work documenting the relevance of the multi-basic cleavage site (MBCS) in the spike (S) protein of SARS-CoV-2 for determining cell tropism and mode of cell entry. The paper describes a number of important findings: 1) That SARS-CoV-2 grown in Vero cells rapidly acquires MBCS mutations, where as virus grown in airway epithelial cells or Vero-TMPRSSR2 cells do not; 2) that deep sequencing is necessary to see mutations emerging that are not apparent in consensus sequence reads; 3) that factors such as fetal calf serum can influence the selection of mutant phenotypes, and 4) that cultures derived from differentiated stem cells can provide reproducible systems for virus culture. Together, the work sets out clear guidelines for the propagation of SARS-CoV-2 to avoid adaptations to laboratory cell-lines/conditions and maintain the authenticity of clinical isolates. The work has relevance to other viruses and the use of permissive transformed cell lines.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

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  2. Trans-ancestry analysis reveals genetic and nongenetic associations with COVID-19 susceptibility and severity

    This article has 35 authors:
    1. Janie F. Shelton
    2. Anjali J. Shastri
    3. Chelsea Ye
    4. Catherine H. Weldon
    5. Teresa Filshtein-Sonmez
    6. Daniella Coker
    7. Antony Symons
    8. Jorge Esparza-Gordillo
    9. The 23andMe COVID-19 Team
    10. Adrian Chubb
    11. Alison Fitch
    12. Alison Kung
    13. Amanda Altman
    14. Andy Kill
    15. Jason Tan
    16. Jeff Pollard
    17. Jey McCreight
    18. Jess Bielenberg
    19. John Matthews
    20. Johnny Lee
    21. Lindsey Tran
    22. Michelle Agee
    23. Monica Royce
    24. Nate Tang
    25. Pooja Gandhi
    26. Raffaello d’Amore
    27. Ruth Tennen
    28. Scott Dvorak
    29. Scott Hadly
    30. Sungmin Park
    31. Taylor Morrow
    32. Trung Le
    33. Yiwen Zheng
    34. Stella Aslibekyan
    35. Adam Auton

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  3. Transferrin receptor is another receptor for SARS-CoV-2 entry

    This article has 16 authors:
    1. Xiaopeng Tang
    2. Mengli Yang
    3. Zilei Duan
    4. Zhiyi Liao
    5. Lei Liu
    6. Ruomei Cheng
    7. Mingqian Fang
    8. Gan Wang
    9. Hongqi Liu
    10. Jingwen Xu
    11. Peter M Kamau
    12. Zhiye Zhang
    13. Lian Yang
    14. Xudong Zhao
    15. Xiaozhong Peng
    16. Ren Lai

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  4. The potential health and economic value of SARS-CoV-2 vaccination alongside physical distancing in the UK: a transmission model-based future scenario analysis and economic evaluation

    This article has 51 authors:
    1. Frank G Sandmann
    2. Nicholas G Davies
    3. Anna Vassall
    4. W John Edmunds
    5. Mark Jit
    6. Fiona Yueqian Sun
    7. C Julian Villabona-Arenas
    8. Emily S Nightingale
    9. Alicia Showering
    10. Gwenan M Knight
    11. Katharine Sherratt
    12. Yang Liu
    13. Kaja Abbas
    14. Sebastian Funk
    15. Akira Endo
    16. Joel Hellewell
    17. Alicia Rosello
    18. Rachel Lowe
    19. Matthew Quaife
    20. Amy Gimma
    21. Oliver Brady
    22. Jack Williams
    23. Simon R Procter
    24. Rosalind M Eggo
    25. Yung-Wai Desmond Chan
    26. James D Munday
    27. Rosanna C Barnard
    28. Georgia R Gore-Langton
    29. Nikos I Bosse
    30. Naomi R Waterlow
    31. Charlie Diamond
    32. Timothy W Russell
    33. Graham Medley
    34. Stefan Flasche
    35. Katherine E Atkins
    36. Kiesha Prem
    37. David Simons
    38. Megan Auzenbergs
    39. Damien C Tully
    40. Christopher I Jarvis
    41. Kevin van Zandvoort
    42. Sam Abbott
    43. Carl A B Pearson
    44. Thibaut Jombart
    45. Sophie R Meakin
    46. Anna M Foss
    47. Adam J Kucharski
    48. Billy J Quilty
    49. Hamish P Gibbs
    50. Samuel Clifford
    51. Petra Klepac

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  5. Major alterations in the mononuclear phagocyte landscape associated with COVID-19 severity

    This article has 92 authors:
    1. Egle Kvedaraite
    2. Laura Hertwig
    3. Indranil Sinha
    4. Andrea Ponzetta
    5. Ida Hed Myrberg
    6. Magda Lourda
    7. Majda Dzidic
    8. Mira Akber
    9. Jonas Klingström
    10. Elin Folkesson
    11. Jagadeeswara Rao Muvva
    12. Puran Chen
    13. Sara Gredmark-Russ
    14. Susanna Brighenti
    15. Anna Norrby-Teglund
    16. Lars I. Eriksson
    17. Olav Rooyackers
    18. Soo Aleman
    19. Kristoffer Strålin
    20. Hans-Gustaf Ljunggren
    21. Florent Ginhoux
    22. Niklas K. Björkström
    23. Jan-Inge Henter
    24. Mattias Svensson
    25. Karolinska KI/K COVID-19 Study Group
    26. John Tyler Sandberg
    27. Helena Bergsten
    28. Niklas K Björkström
    29. Susanna Brighenti
    30. Marcus Buggert
    31. Marta Butrym
    32. Benedict J Chambers
    33. Puran Chen
    34. Martin Cornillet
    35. Angelica Cuapio
    36. Isabel Diaz Lozano
    37. Majda Dzidic
    38. Johanna Emgård
    39. Malin Flodström-Tullberg
    40. Jean-Baptiste Gorin
    41. Sara Gredmark-Russ
    42. Alvaro Haroun-Izquierdo
    43. Laura Hertwig
    44. Sadaf Kalsum
    45. Jonas Klingström
    46. Efthymia Kokkinou
    47. Egle Kvedaraite
    48. Hans-Gustaf Ljunggren
    49. Nicole Marquardt
    50. Magdalini Lourda
    51. Kimia T Maleki
    52. Karl-Johan Malmberg
    53. Jakob Michaëlsson
    54. Jenny Mjösberg
    55. Kirsten Moll
    56. Jagadeeswara Rao Muvva
    57. Anna Norrby-Teglund
    58. Laura M Palma Medina
    59. Tiphaine Parrot
    60. Lena Radler
    61. Emma Ringqvist
    62. Johan K Sandberg
    63. Takuya Sekine
    64. Tea Soini
    65. Mattias Svensson
    66. Janne Tynell
    67. Andreas von Kries
    68. David Wullimann
    69. André Perez-Potti
    70. Olga Rivera-Ballesteros
    71. Christopher Maucourant
    72. Renata Varnaite
    73. Mira Akber
    74. Lena Berglin
    75. Demi Brownlie
    76. Marco Giulio Loreti
    77. Ebba Sohlberg
    78. Tobias Kammann
    79. Elisabeth Henriksson
    80. Kristoffer Strålin
    81. Soo Aleman
    82. Anders Sönnerborg
    83. Lena Dillner
    84. Anna Färnert
    85. Hedvig Glans
    86. Pontus Nauclér
    87. Olav Rooyackers
    88. Johan Mårtensson
    89. Lars I Eriksson
    90. Björn P Persson
    91. Jonathan Grip
    92. Christian Unge

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  6. Comprehensive analysis of T cell immunodominance and immunoprevalence of SARS-CoV-2 epitopes in COVID-19 cases

    This article has 22 authors:
    1. Alison Tarke
    2. John Sidney
    3. Conner K. Kidd
    4. Jennifer M. Dan
    5. Sydney I. Ramirez
    6. Esther Dawen Yu
    7. Jose Mateus
    8. Ricardo da Silva Antunes
    9. Erin Moore
    10. Paul Rubiro
    11. Nils Methot
    12. Elizabeth Phillips
    13. Simon Mallal
    14. April Frazier
    15. Stephen A. Rawlings
    16. Jason A. Greenbaum
    17. Bjoern Peters
    18. Davey M. Smith
    19. Shane Crotty
    20. Daniela Weiskopf
    21. Alba Grifoni
    22. Alessandro Sette

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