Synthetic Biology

Enhanced Cas12a multi-gene regulation using a CRISPR array separator

1. Jens P Magnusson
2. Antonio Ray Rios
3. Lingling Wu
4. Lei S Qi

• Curated by eLife

  Evaluation Summary:

  This manuscript is of broad interest to those performing multiplexed genome engineering and related applications with CRISPR Cas12a technologies. While the proposed use of synSeparators is promising, the paper would benefit from further investigation of the mechanism by which synSeparators function to promote Cas12a activity. Additional data would be required to support the current conclusions regarding the generalizability of the findings.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

Reviewed by eLife

Mapping the functional landscape of the receptor binding domain of T7 bacteriophage by deep mutational scanning

1. Phil Huss
2. Anthony Meger
3. Megan Leander
4. Kyle Nishikawa
5. Srivatsan Raman

Reviewed by eLife

Converting endogenous genes of the malaria mosquito into simple non-autonomous gene drives for population replacement

1. Astrid Hoermann
2. Sofia Tapanelli
3. Paolo Capriotti
4. Giuseppe Del Corsano
5. Ellen KG Masters
6. Tibebu Habtewold
7. George K Christophides
8. Nikolai Windbichler

Reviewed by eLife