@kenton_swartz's saved articles

The M1 and pre-M1 segments contribute differently to ion selectivity in ASICs and ENaCs

1. Zeshan P. Sheikh
2. Matthias Wulf
3. Søren Friis
4. Mike Althaus
5. Timothy Lynagh
6. Stephan A. Pless

The action of Con-ikot-ikot toxin on single AMPA-type glutamate receptors

1. Jelena Baranovic
2. Sebastian Braunbeck
3. Nikolai Zaki
4. Sonja Minniberger
5. Miriam Chebli
6. Andrew J.R. Plested

Topography and motion of acid-sensing ion channel intracellular domains

1. Tyler Couch
2. Kyle D Berger
3. Dana L Kneisley
4. Tyler W McCullock
5. Paul Kammermeier
6. David M Maclean

• Curated by eLife

  Evaluation Summary:

  This is a rigorous and clearly written paper that provides quantitative data for the scope of intracellular signalling by ASIC channels. These channels are involved in pain signalling and other processes, and apparently can couple to intracellular pathways independent of ion flow. Here the authors measure the movements of the unstructured intracellular parts of ASIC using fluorescence spectroscopy coupled to functional measurements.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1, #2, and #3 agreed to share their names with the authors.)

Reviewed by eLife

Structures of the TRPM5 channel elucidate mechanisms of activation and inhibition

1. Zheng Ruan
2. Emery Haley
3. Ian J. Orozco
4. Mark Sabat
5. Richard Myers
6. Rebecca Roth
7. Juan Du
8. Wei Lü

P2X2 receptor subunit interfaces are missense variant hotspots, where mutations tend to increase apparent ATP affinity

1. Federica Gasparri
2. Debayan Sarkar
3. Sarune Bielickaite
4. Mette Homann Poulsen
5. Alexander Sebastian Hauser
6. Stephan Alexander Pless

Piezo1 ion channels inherently function as independent mechanotransducers

1. Amanda H Lewis
2. Jörg Grandl

• Curated by eLife

  Evaluation Summary:

  The work, when completed, will provide important mechanistic detail on how Piezo channels, the most important and versatile mechanoreceptor molecules, functionally interact in the plane of the plasma membrane. It will be of interest to the field of mechanobiology and sensory mechanotransduction.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

TRPC3 and NALCN channels drive pacemaking in substantia nigra dopaminergic neurons

1. Ki Bum Um
2. Suyun Hahn
3. So Woon Kim
4. Yoon Je Lee
5. Lutz Birnbaumer
6. Hyun Jin Kim
7. Myoung Kyu Park

• Curated by eLife

  Evaluation Summary:

  This work clearly demonstrates an important role for two specific sodium-permeable ion channels for maintaining the pacemaker-like firing of midbrain dopamine neurons. These neurons have a key role in motivation, reinforcement and locomotion, and have been implicated in Parkinson's disease and multiple neuropsychiatric disorders. The authors also find that the regular firing of these cells is robustly maintained even when one of the two channels is knocked out, through upregulation of the level of the other channel.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife

Analysis of the mechanosensor channel functionality of TACAN

1. Yiming Niu
2. Xiao Tao
3. George Vaisey
4. Paul Dominic B Olinares
5. Hanan Alwaseem
6. Brian T Chait
7. Roderick MacKinnon

TMEM120A contains a specific coenzyme A-binding site and might not mediate poking- or stretch-induced channel activities in cells

1. Yao Rong
2. Jinghui Jiang
3. Yiwei Gao
4. Jianli Guo
5. Danfeng Song
6. Wenhao Liu
7. Mingmin Zhang
8. Yan Zhao
9. Bailong Xiao
10. Zhenfeng Liu

• Curated by eLife

  Evaluation Summary:

  The paper describes novel structures of a protein recently reported to function as a mechanosensitive ion channel. Surprisingly, the structures and functional data rather support the formerly suggested role of this protein in lipid metabolism. The paper is of relevance for ion channel field and for those interested in fatty acid metabolism.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

Conformational decoupling in acid-sensing ion channels uncovers mechanism and stoichiometry of PcTx1-mediated inhibition

1. Stephanie A Heusser
2. Christian B Borg
3. Janne M Colding
4. Stephan A Pless

• Curated by eLife

  Evaluation Summary:

  This work provides direct evidence that PcTx1, a modulator commonly used to study acid-sensing ion channels, induces a conformational change that persists long after an effect on the channel activity has dissipated. The data support this central claim of the paper and invite future investigation of the precise mechanism. The work is of general interest to those studying ion channel biophysics and pharmacology and is a fine example of the power of combined functional and fluorescence measurements.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

Reviewed by eLife

Structural determinants of acid-sensing ion channel potentiation by single chain lipids

1. Robert C. Klipp
2. John R. Bankston

Cryo-EM structures of human TMEM120A and TMEM120B

1. Meng Ke
2. Yue Yu
3. Changjian Zhao
4. Shirong Lai
5. Qiang Su
6. Weidan Yuan
7. Lina Yang
8. Dong Deng
9. Kun Wu
10. Weizheng Zeng
11. Jia Geng
12. Jianping Wu
13. Zhen Yan

TMEM120A/TACAN inhibits mechanically activated PIEZO2 channels

1. John Smith Del Rosario
2. Matthew Gabrielle
3. Yevgen Yudin
4. Tibor Rohacs

• Curated by eLife

  Evaluation Summary:

  This manuscript will be of interest people studying mechanosensation (particularly touch and pain sensation) and ion channels. The authors use a combination of electrophysiology and imaging to provide evidence that the force-gated ion channel Piezo2 is negatively regulation by the enzyme Tmem120A/Tacan. The results from the heterologous expression aspects of study are relatively robust and may have potential impact on the field. The physiological relevance of these findings await further investigation.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Structural insights into the Venus flytrap mechanosensitive ion channel Flycatcher1

1. Sebastian Jojoa-Cruz
2. Kei Saotome
3. Che Chun Alex Tsui
4. Wen-Hsin Lee
5. Mark S. P. Sansom
6. Swetha E. Murthy
7. Ardem Patapoutian
8. Andrew B. Ward

The versatile regulation of K2P channels by polyanionic lipids of the phosphoinositide and fatty acid metabolism

1. Elena B. Riel
2. Björn C. Jürs
3. Sönke Cordeiro
4. Marianne Musinszki
5. Marcus Schewe
6. Thomas Baukrowitz

Reviewed by Biophysics Colab

Single-molecule imaging with cell-derived nanovesicles reveals early binding dynamics at a cyclic nucleotide-gated ion channel

1. Vishal R. Patel
2. Arturo M. Salinas
3. Darong Qi
4. Shipra Gupta
5. David J. Sidote
6. Marcel P. Goldschen-Ohm

• Curated by Biophysics Colab

  Endorsement statement (30 August 2021)

  The preprint by Patel et al. describes the development of a single molecule approach for studying individual ligand binding events in membrane proteins within native lipid environments. The approach represents an elegant way to investigate the dynamics of ligand binding, and potential relationships with conformational changes, in molecules embedded within physiological membranes. The work makes an important contribution that will be of interest to scientists working on molecular mechanisms in ion channels and other membrane proteins.

  (This endorsement by Biophysics Colab refers to version 2 of this preprint, which has been revised in response to peer review of version 1.)

Reviewed by Biophysics Colab

An Advanced Automated Patch Clamp Protocol Design to Investigate Drug—Ion Channel Binding Dynamics

1. Peter Lukacs
2. Krisztina Pesti
3. Mátyás C. Földi
4. Katalin Zboray
5. Adam V. Toth
6. Gábor Papp
7. Arpad Mike

The molecular appearance of native TRPM7 channel complexes identified by high-resolution proteomics

1. Astrid Kollewe
2. Vladimir Chubanov
3. Fong Tsuen Tseung
4. Leonor Correia
5. Eva Schmidt
6. Anna Rössig
7. Susanna Zierler
8. Alexander Haupt
9. Catrin Swantje Müller
10. Wolfgang Bildl
11. Uwe Schulte
12. Annette Nicke
13. Bernd Fakler
14. Thomas Gudermann

Effects of ionic strength on gating and permeation of TREK-2 K2P channels

1. Linus J. Conrad
2. Peter Proks
3. Stephen J. Tucker

Discovery and characterization of Hv1-type proton channels in reef-building corals

1. Gisela Rangel-Yescas
2. Cecilia Cervantes
3. Miguel A Cervantes-Rocha
4. Esteban Suárez-Delgado
5. Anastazia T Banaszak
6. Ernesto Maldonado
7. Ian Scott Ramsey
8. Tamara Rosenbaum
9. Leon D Islas

• Curated by eLife

  Evaluation Summary:

  This paper will be of interest both to marine biologists and to biophysicists studying voltage-gated proton channels. It describes cloning and full biophysical characterization of the first ion channel ever identified in reef-building coral species, and develops a mechanistic model for understanding regulation of voltage-gated proton channels.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife