@johnl's saved articles

eLife assessment

This is a valuable paper that identifies a potential challenge for embryos during fertilization: holding sperm contents in the fertilized embryos away from the oocyte meiotic spindle so that they don't get ejected into the polar body during meiotic chromosome segregation. The authors identify proteins involved in cytoplasmic streaming and maintaining the grouping of paternal organelles as being critical for this process. There remain minor weaknesses in the data presented but the paper provides solid evidence for the majority of its claims, and while the findings may pertain to a narrow audience the tools used and basic characterization shown will likely be relied upon by many in the community and therefore is of high value.

eLife assessment

This is a potentially valuable study suggesting that neuronal-specific loss of function of the RNA splicing factor Ptbp1 in striatal neurons induces dopaminergic markers and alleviates motor defects in a 6-hydroxydopamine (6-OHDA) mouse model of Parkinson's Disease. If properly replicated, the claims of the manuscript are remarkable and identify a straightforward mechanism with therapeutic relevance for the treatment of motor deficits in Parkinson's Disease. However, while the rescue of motor deficits with Ptbp1 manipulation is solid, the strength of the evidence supporting the induction of a dopaminergic neuronal identity is incomplete. The study nevertheless addresses recent controversial literature on cell reprogramming in Parkinson's Disease and will be of interest to researchers with a focus on the application of gene therapy to rescue neurodegeneration.