Molecular Biology

Tetrahydroxanthohumol, a xanthohumol derivative, attenuates high-fat diet-induced hepatic steatosis by antagonizing PPARγ

1. Yang Zhang
2. Gerd Bobe
3. Cristobal L Miranda
4. Malcolm B Lowry
5. Victor L Hsu
6. Christiane V Lohr
7. Carmen P Wong
8. Donald B Jump
9. Matthew M Robinson
10. Thomas J Sharpton
11. Claudia S Maier
12. Jan F Stevens
13. Adrian F Gombart

• Curated by eLife

  Evaluation Summary:

  This is a comprehensive study of the effect of xanthohumol and TXN, a xanthohumol derivative, on different pathologies related to the metabolic syndrome. It clearly shows the therapeutic potential of these substances, which has a high translational potential since currently, there are is a lack of effective therapies.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

No Evidence of Coronaviruses or Other Potentially Zoonotic Viruses in Sunda pangolins (Manis javanica) Entering the Wildlife Trade via Malaysia

1. Jimmy Lee
2. Tom Hughes
3. Mei-Ho Lee
4. Hume Field
5. Jeffrine Japning Rovie-Ryan
6. Frankie Thomas Sitam
7. Symphorosa Sipangkui
8. Senthilvel K. S. S. Nathan
9. Diana Ramirez
10. Subbiah Vijay Kumar
11. Helen Lasimbang
12. Jonathan H. Epstein
13. Peter Daszak

Reviewed by ScreenIT, NCRC

Widespread premature transcription termination of Arabidopsis thaliana NLR genes by the spen protein FPA

1. Matthew T Parker
2. Katarzyna Knop
3. Vasiliki Zacharaki
4. Anna V Sherwood
5. Daniel Tomé
6. Xuhong Yu
7. Pascal GP Martin
8. Jim Beynon
9. Scott D Michaels
10. Geoffrey J Barton
11. Gordon G Simpson

• Curated by eLife

  Evaluation Summary:

  In this study, the authors examined the function of the RNA-binding protein FPA through analyzing its protein interactome and its global impact on gene expression using a combined approach of Nanopore DRS, Helicos DRS, and short-read Illumina RNA-Seq. The combined datasets and new computational approaches developed by the authors permitted them to identify the predominant role of FPA in promoting poly(A) site choice. The authors further revealed that FPA mediates widespread premature cleavage and polyadenylation of transcripts of NLR genes, important plant immune regulators. Overall, this study suggests that control of transcription termination processes mediated by FPA provides an additional layer of the regulatory dynamics of NLRs in plant immune responses.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #3 agreed to share their names with the authors.)

Reviewed by eLife

Surprising phenotypic diversity of cancer-associated mutations of Gly 34 in the histone H3 tail

1. Brandon R Lowe
2. Rajesh K Yadav
3. Ryan A Henry
4. Patrick Schreiner
5. Atsushi Matsuda
6. Alfonso G Fernandez
7. David Finkelstein
8. Margaret Campbell
9. Satish Kallappagoudar
10. Carolyn M Jablonowski
11. Andrew J Andrews
12. Yasushi Hiraoka
13. Janet F Partridge

Reviewed by eLife

PIE-1 promotes SUMOylation and activation of HDAC1 during the C. elegans oogenesis

1. Heesun Kim
2. Yue-He Ding
3. Shan Lu
4. Mei-Qing Zuo
5. Darryl Conte
6. Meng-Qiu Dong
7. Craig C. Mello

• Curated by eLife

  Summary: In this paper you describe experiments showing that PIE-1 is sumoylated at K68, and that K68 sumoylation plays a role in PIE-1 interaction with HDA-1 and its sumoylation, which leads to its activation. The reviewers found the sumoylation dependence of PIE-1 function in piRNA silencing to be of interest, but raised major issues that need to be addressed. In particular, more mechanistic insights into how sumoylation of PIE-1 at K68 enhances HDA-1 sumoylation and regulation are required.

  This is a co-submission with the manuscript https://www.biorxiv.org/content/10.1101/2020.08.17.254466v2

Reviewed by eLife

HDAC1 SUMOylation promotes Argonaute-directed transcriptional silencing in C. elegans

1. Heesun Kim
2. Yue-He Ding
3. Gangming Zhang
4. Yong-Hong Yan
5. Darryl Conte
6. Meng-Qiu Dong
7. Craig C Mello

• Curated by eLife

  Summary: In this paper, your studies showed that sumoylation of HDA-1, a type 1 HDAC, at two C-terminal Lys residues plays a role in establishing transcriptional silencing of piRNA-regulated genes in C. elegans through enhanced NuRD complex interaction and histone H3 deacetylation. The reviewers all found the link between HDA-1 sumoylation and silencing to be interesting, but raised a number of issues that need to be addressed.

  This is a co-submission with the manuscript https://www.biorxiv.org/content/10.1101/2020.08.17.254466v2

Reviewed by eLife

Fine-Tuning of Piezo1 Expression and Activity Ensures Efficient Myoblast Fusion during Skeletal Myogenesis

1. Huascar Pedro Ortuste Quiroga
2. Massimo Ganassi
3. Shingo Yokoyama
4. Kodai Nakamura
5. Tomohiro Yamashita
6. Daniel Raimbach
7. Arisa Hagiwara
8. Oscar Harrington
9. Jodie Breach-Teji
10. Atsushi Asakura
11. Yoshiro Suzuki
12. Makoto Tominaga
13. Peter S. Zammit
14. Katsumasa Goto

Reviewed by eLife

Basis of specificity for a conserved and promiscuous chromatin remodeling protein

1. Drake A Donovan
2. Johnathan G Crandall
3. Vi N Truong
4. Abigail L Vaaler
5. Thomas B Bailey
6. Devin Dinwiddie
7. Orion GB Banks
8. Laura E McKnight
9. Jeffrey N McKnight

Reviewed by eLife

SUV39 SET domains mediate crosstalk of heterochromatic histone marks

1. Alessandro Stirpe
2. Nora Guidotti
3. Sarah J Northall
4. Sinan Kilic
5. Alexandre Hainard
6. Oscar Vadas
7. Beat Fierz
8. Thomas Schalch

Reviewed by eLife, preLights

DHX30 Coordinates Cytoplasmic Translation and Mitochondrial Function Contributing to Cancer Cell Survival

1. Bartolomeo Bosco
2. Annalisa Rossi
3. Dario Rizzotto
4. Meriem Hadjer Hamadou
5. Alessandra Bisio
6. Sebastiano Giorgetta
7. Alicia Perzolli
8. Francesco Bonollo
9. Angeline Gaucherot
10. Frédéric Catez
11. Jean-Jacques Diaz
12. Erik Dassi
13. Alberto Inga

Reviewed by eLife