Microbiology

Dissecting the invasion of Galleria mellonella by Yersinia enterocolitica reveals metabolic adaptations and a role of a phage lysis cassette in insect killing

1. Philipp-Albert Sänger
2. Stefanie Wagner
3. Elisabeth M. Liebler-Tenorio
4. Thilo M. Fuchs

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Convergence of two global regulators to coordinate expression of essential virulence determinants of Mycobacterium tuberculosis

1. Hina Khan
2. Partha Paul
3. Ritesh Rajesh Sevalkar
4. Sangita Kachhap
5. Balvinder Singh
6. Dibyendu Sarkar

• Curated by eLife

  Evaluation Summary:

  This paper will be of broad interest to those working on the regulation of gene expression and mycobacteria as it deals with the collaboration of two important transcription regulators. A combination of experiments indicates how a complex of two regulators selectively turns on gene expression of a few genes in intracellular pathogen Mycobacterium tuberculosis.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

Temporal and thermal profiling of the Toxoplasma proteome implicates parasite Protein Phosphatase 1 in the regulation of Ca2+-responsive pathways

1. Alice L Herneisen
2. Zhu-Hong Li
3. Alex W Chan
4. Silvia NJ Moreno
5. Sebastian Lourido

• Curated by eLife

  Evaluation Summary:

  Herneisen et al provide a comprehensive and thorough exploration of Ca2+ responsive changes in the Toxoplasma proteome and the resulting phosphorylation events during the transition from intracellular residing parasites to egress from the host cell. Furthermore, a novel temperature stability profiling method of all proteins responding to Ca2+ concentration with a change in stability is a novel applicable tool that here is used to map Ca2+-responsive proteins in the parasites. They provide a compelling analysis of the complex data and carefully validate their findings using genetics and cell biology. This work is of the highest quality in the field.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

An Atypical F-Actin Capping Protein Modulates Cytoskeleton Behaviors Crucial for Trichomonas vaginalis Colonization

1. Kai-Hsuan Wang
2. Jing-Yang Chang
3. Fu-An Li
4. Kuan-Yi Wu
5. Shu-Hao Hsu
6. Yen-Ju Chen
7. Tse-Ling Chu
8. Jessica Lin
9. Hong-Ming Hsu

• Curated by eLife

  Evaluation Summary:

  Trichomonas vaginalis is a sexually transmitted protist that colonizes its host by transitioning from flagellar locomotion to an adherent ameboid movement. In this manuscript, Wang and coauthors use a wide range of experimental approaches to investigate the function of a novel actin capping protein in T. vaginalis cytoadherence and cell motility. The work provides an intriguing example of how an unusual capping protein may impact cytoskeletal organization and cell behavior.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 and Reviewer #3 agreed to share their names with the authors.)

Reviewed by eLife

The evolution of a counter-defense mechanism in a virus constrains its host range

1. Sriram Srikant
2. Chantal K Guegler
3. Michael T Laub

• Curated by eLife

  Evaluation Summary:

  This manuscript will be of interest to researchers in the phage-microbial host interaction field. Notably, the interplay between bacteria and their viral predators has regained broad interest in recent years given the discovery of numerous innate immunity-like phage defense systems. The identification of phage-mediated counter-defense strategies is therefore not only of prime importance for our basic understanding of predator-prey arms races but also for medical applications such as phage therapy.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

c-Myc plays a key role in IFN-γ-induced persistence of Chlamydia trachomatis

1. Nadine Vollmuth
2. Lisa Schlicker
3. Yongxia Guo
4. Pargev Hovhannisyan
5. Sudha Janaki-Raman
6. Naziia Kurmasheva
7. Werner Schmitz
8. Almut Schulze
9. Kathrin Stelzner
10. Karthika Rajeeve
11. Thomas Rudel

• Curated by eLife

  Evaluation Summary:

  This paper will be of interest to scientists working to understand Chlamydia trachomatis persistence, and host pathogen interaction in general. The authors report the surprising observation that the mechanism of restriction of bacterial growth is through the inhibition of c-Myc signaling by IFNg as opposed to IDO-dependent depletion of tryptophan levels, as had been previously suggested.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Host tropism determination by convergent evolution of immunological evasion in the Lyme disease system

1. Thomas M. Hart
2. Alan P. Dupuis
3. Danielle M. Tufts
4. Anna M. Blom
5. Simon R. Starkey
6. Ryan O. M. Rego
7. Sanjay Ram
8. Peter Kraiczy
9. Laura D. Kramer
10. Maria A. Diuk-Wasser
11. Sergios-Orestis Kolokotronis
12. Yi-Pin Lin

• Curated by eLife

  Evaluation Summary:

  This work builds on previous work by the same team, demonstrating that the bacterial protein CspA, which inactivates host complement by binding to the host complement inhibitor FH, is a determinant of host range for the Lyme disease bacterium. Additionally, the authors present phylogenetic analysis of CspA and related protein sequences, which supports the hypothesis that inactivation of host complement has evolved independently in three bacterial genospecies.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife

Distinct regions of H. pylori’s bactofilin CcmA regulate protein–protein interactions to control helical cell shape

1. Sophie R Sichel
2. Benjamin P Bratton
3. Nina R Salama

• Curated by eLife

  Evaluation Summary:

  The helical shape of the bacterial pathogen Helicobacter pylori is important for its ability to colonize the human gut. Building on previous work identifying a complex of proteins required for generating helicity, this study focuses on the molecular mechanisms by which this complex modulates cell shape. Based on results from genetic, cytological, and pull-down experiments, the authors propose that one member of the complex, the bactofilin CcmA, interacts with two other complex members to generate helicity through a combination of cell wall synthesis and degradation. While data is supportive of this idea, the conclusions of the study require additional experimental support to rule out competing models.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife

Cell detoxification of secondary metabolites by P4-ATPase-mediated vesicle transport

1. Yujie Li
2. Hui Ren
3. Fanlong Wang
4. Jianjun Chen
5. Lian Ma
6. Yang Chen
7. Xianbi Li
8. Yanhua Fan
9. Dan Jin
10. Lei Hou
11. Yonghong Zhou
12. Nemat O Keyhani
13. Yan Pei

• Curated by eLife

  Evaluation Summary:

  In this manuscript, the authors focus on the fungus B. bassiana, which is resistant to the toxin cyclosporine A. Through a mutant screen, the authors identify the key gene that mediates the sequestration of the toxin in vacuoles. They further show that this gene can be transferred to a distinct fungus and also to plants to protect against a toxin-producing fungal pathogen. Therefore, this work may lead to novel disease control strategies against fungal pathogens.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Recognition of copy-back defective interfering rabies virus genomes by RIG-I triggers the antiviral response against vaccine strains

1. Wahiba Aouadi
2. Valérie Najburg
3. Rachel Legendre
4. Hugo Varet
5. Lauriane Kergoat
6. Frédéric Tangy
7. Florence Larrous
8. Anastassia V. Komarova
9. Hervé Bourhy

Reviewed by Review Commons