Microbiology

Effects of common mutations in the SARS-CoV-2 Spike RBD and its ligand, the human ACE2 receptor on binding affinity and kinetics

1. Michael I Barton
2. Stuart A MacGowan
3. Mikhail A Kutuzov
4. Omer Dushek
5. Geoffrey John Barton
6. P Anton van der Merwe

• Curated by eLife

  Evaluation Summary:

  This manuscript is of interest to virologists working on SARS-CoV-2, as well as biochemists and biophysicists who perform binding experiments with surface plasmon resonance (SPR), as it provides detailed affinity and kinetics analysis of the effect of mutations in variants of concern in the SARS-CoV-2 receptor binding domain with receptor ACE2 and two 'common' mutations in ACE2.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife, ScreenIT

Allicin inhibits SARS-CoV-2 replication and abrogates the antiviral host response in the Calu-3 proteome

1. Kirstin Mösbauer
2. Verena Nadin Fritsch
3. Lorenz Adrian
4. Jörg Bernhardt
5. Martin Clemens Horst Gruhlke
6. Alan John Slusarenko
7. Daniela Niemeyer
8. Haike Antelmann

Reviewed by ScreenIT

A covariation analysis reveals elements of selectivity in quorum sensing systems

1. Samantha Wellington Miranda
2. Qian Cong
3. Amy L Schaefer
4. Emily Kenna MacLeod
5. Angelina Zimenko
6. David Baker
7. E Peter Greenberg

• Curated by eLife

  Evaluation Summary:

  Acyl-homoserine lactone (AHL) based quorum sensing systems are an important form of intercellular communication in bacteria. These systems, minimally comprised of a synthase and a receptor, often involve different types of AHLs. This paper demonstrates that amino acids in the active site of the synthase and the binding site of the receptor have co-evolved to provide specificity for different signaling molecules, even though the synthase and receptor do not directly interact. This type of information could potentially be used to rationally engineer synthases and receptors with different specificities, as well as predict the specificities of uncharacterized synthases and receptors.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

Reviewed by eLife

A novel mechanosensitive channel controls osmoregulation, differentiation and infectivity in Trypanosoma cruzi

1. N Dave
2. U Cetiner
3. D Arroyo
4. J Fonbuena
5. M Tiwari
6. P Barrera
7. N Lander
8. A Anishkin
9. S Sukharev
10. V Jimenez

• Curated by eLife

  Evaluation Summary:

  The authors found a mechanosensitive channel gene in T. cruzi, and aimed to characterize its functions. The authors provide conclusive evidence that TcMscS is a mechanosensitive channel. They also show that TcMscS has additional roles outside of mechanosensation, likely playing a role in the infectivity of T. cruzi.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife

Common Mechanism of SARS-CoV and SARS-CoV-2 Pathogenesis across Species

1. Alexandra Schäfer
2. Lisa E. Gralinski
3. Sarah R. Leist
4. Emma S. Winkler
5. Brea K. Hampton
6. Michael A. Mooney
7. Kara L. Jensen
8. Rachel L. Graham
9. Sudhakar Agnihothram
10. Sophia Jeng
11. Steven Chamberlin
12. Timothy A. Bell
13. D. Trevor Scobey
14. Laura A. VanBlargan
15. Larissa B. Thackray
16. Pablo Hock
17. Darla R. Miller
18. Ginger D. Shaw
19. Fernando Pardo Manuel de Villena
20. Shannon K. McWeeney
21. Stephanie A. Montgomery
22. Michael S. Diamond
23. Mark T. Heise
24. Vineet D. Menachery
25. Martin T. Ferris
26. Ralph S. Baric

Reviewed by ScreenIT

Glucosidase inhibitors suppress SARS-CoV-2 in tissue culture and may potentiate

1. Hanako Reyes
2. Yanming Du
3. Tianlun Zhou
4. Xuping Xie
5. Pei Yong Shi
6. Susan Weiss
7. Timothy M. Block

Reviewed by ScreenIT

Siglec-1 on dendritic cells mediates SARS-CoV-2 trans -infection of target cells while on macrophages triggers proinflammatory responses

1. Daniel Perez-Zsolt
2. Jordana Muñoz-Basagoiti
3. Jordi Rodon
4. Marc Elousa
5. Dàlia Raïch-Regué
6. Cristina Risco
7. Martin Sachse
8. Maria Pino
9. Sanjeev Gumber
10. Mirko Paiardini
11. Jakub Chojnacki
12. Itziar Erkizia
13. Xabier Muñiz
14. Ester Ballana
15. Eva Riveira-Muñoz
16. Marc Noguera
17. Roger Paredes
18. Benjamin Trinité
19. Ferran Tarrés-Freixas
20. Ignacio Blanco
21. Victor Guallar
22. Jorge Carrillo
23. Julià Blanco
24. Amalio Telenti
25. Holger Heyn
26. Joaquim Segalés
27. Bonaventura Clotet
28. Javier Martinez-Picado
29. Júlia Vergara-Alert
30. Nuria Izquierdo-Useros

Reviewed by ScreenIT

Analysis of the Role of N-Linked Glycosylation in Cell Surface Expression, Function, and Binding Properties of SARS-CoV-2 Receptor ACE2

1. Raymond Rowland
2. Alberto Brandariz-Nuñez

Reviewed by ScreenIT

Modeling SARS-CoV-2 and influenza infections and antiviral treatments in human lung epithelial tissue equivalents

1. Hoda Zarkoob
2. Anna Allué-Guardia
3. Yu-Chi Chen
4. Andreu Garcia-Vilanova
5. Olive Jung
6. Steven Coon
7. Min Jae Song
8. Jun-Gyu Park
9. Fatai Oladunni
10. Jesse Miller
11. Yen-Ting Tung
12. Ivan Kosik
13. David Schultz
14. James Iben
15. Tianwei Li
16. Jiaqi Fu
17. Forbes D. Porter
18. Jonathan Yewdell
19. Luis Martinez-Sobrido
20. Sara Cherry
21. Jordi B. Torrelles
22. Marc Ferrer
23. Emily M. Lee

Reviewed by ScreenIT

Viral shedding and transmission after natural infection and vaccination in an animal model of SARS-CoV-2 propagation

1. Caroline J. Zeiss
2. Jennifer L. Asher
3. Brent Vander Wyk
4. Heather G. Allore
5. Susan Compton

Reviewed by ScreenIT