Genetics

Deregulations of miR‐1 and its target Multiplexin promote dilated cardiomyopathy associated with myotonic dystrophy type 1

1. Anissa Souidi
2. Masayuki Nakamori
3. Monika Zmojdzian
4. Teresa Jagla
5. Yoan Renaud
6. Krzysztof Jagla

Reviewed by Review Commons

A novel overlapping gene azyx-1 affects the translation of zyxin in C. elegans

1. Bhavesh S. Parmar
2. Ellen Geens
3. Elke Vandewyer
4. Amanda Kieswetter
5. Christina Ludwig
6. Liesbet Temmerman

Reviewed by Review Commons

Exploiting the mediating role of the metabolome to unravel transcript-to-phenotype associations

1. Chiara Auwerx
2. Marie C Sadler
3. Tristan Woh
4. Alexandre Reymond
5. Zoltán Kutalik
6. Eleonora Porcu

• Curated by eLife

  eLife assessment

  Auwerx and colleagues take a new approach to mine large datasets of the intermediary molecular data between GWAS and phenotype, touncover molecular mechanisms that lead from a GWAS hit to a phenotypic effect. The approach should be of great use to all (human) geneticists. Revisions are necessary to ensure that the significant findings from this approach are understood by the bioinformatic community and that these methods can be applied generally, given that the paper's main novelty is in its approach to mine large datasets, rather than a specific, key molecular finding.

Reviewed by eLife

Relating pathogenic loss-of-function mutations in humans to their evolutionary fitness costs

1. Ipsita Agarwal
2. Zachary L Fuller
3. Simon R Myers
4. Molly Przeworski

• Curated by eLife

  eLife assessment

  This paper directly estimates the fitness cost of loss-of-function mutations in almost every gene in the human genome, providing an interpretable measure of the severity of mutations. The authors then compare datasets of presumably healthy individuals and individuals affected by severe complex disorders or genetic disorders, finding enrichment of de novo loss-of-function mutations in highly constrained genes among probands alongside other illuminating results. This important study will be useful to researchers interested in interpreting and prioritizing disease-causing mutations and in the process of human evolution. Overall, the approach is elegant and the results are of high quality and compelling.

Reviewed by eLife

Single-cell RNA sequencing identifies regulators of differentiation and nutritional cues in Drosophila female germ cells

1. Zhipeng Sun
2. Todd G. Nystul
3. Guohua Zhong

Reviewed by Review Commons

Methylglyoxal-derived hydroimidazolone, MG-H1, increases food intake by altering tyramine signaling via the GATA transcription factor ELT-3 in Caenorhabditis elegans

1. Muniesh Muthaiyan Shanmugam
2. Jyotiska Chaudhuri
3. Durai Sellegounder
4. Amit Kumar Sahu
5. Sanjib Guha
6. Manish Chamoli
7. Brian Hodge
8. Neelanjan Bose
9. Charis Amber
10. Dominique O Farrera
11. Gordon Lithgow
12. Richmond Sarpong
13. James J Galligan
14. Pankaj Kapahi

• Curated by eLife

  eLife assessment

  The work, which examines how Advanced Glycation End-products (AGEs), commonly found in processed and other cooked foods, affect eating behavior and signaling in the nematode C. elegans, is in a fundamentally important area of research with clear translational potential for humans. Some aspects of the manuscript are compelling, including the well-characterized assays on food intake, while other aspects are still incomplete, such as the mechanistic work on the neural network responsible for the response to AGEs.

Reviewed by eLife

Efficient knock-in method enabling lineage tracing in zebrafish

1. Jiarui Mi
2. Olov Andersson

Reviewed by Review Commons

Evidence for RNA or protein transport from somatic tissues to the male reproductive tract in mouse

1. Vera Rinaldi
2. Kathleen Messemer
3. Kathleen Desevin
4. Fengyun Sun
5. Bethany C Berry
6. Shweta Kukreja
7. Andrew R Tapper
8. Amy J Wagers
9. Oliver J Rando

• Curated by eLife

  eLife assessment

  This manuscript reports data consistent with a new and unanticipated phenomenon: that Cre or its mRNA may be transmitted between tissues in the mouse and that the male reproductive tract (epididymis) appears to be the most common target of such transported molecules. The data serve as a timely warning to mouse researchers about an unexpected complication of Cre-mediated gene manipulation.

Reviewed by eLife

Drosophila pVALIUM10 TRiP RNAi lines cause undesired silencing of Gateway-based transgenes

1. Dimitrije Stanković
2. Gábor Csordás
3. Mirka Uhlirova

Reviewed by Review Commons

CIRKO: A chemical-induced reversible gene knockout system for studying gene function in situ

1. Hui Shi
2. Qin Jin
3. Fangbing Chen
4. Zhen Ouyang
5. Shixue Gou
6. Xiaoyi Liu
7. Lei Li
8. Shuangshuang Mu
9. Chengdan Lai
10. Quanjun Zhang
11. Yinghua Ye
12. Kepin Wang
13. Liangxue Lai

• Curated by eLife

  eLife assessment

  Conditional deletion and reactivation of a gene in situ remain challenging, and this study therefore addresses a gap in the genetic tool box. The authors introduce a reversible conditional gene inactivation and reactivation method using sequential expression of recombinases, with doxycycline treatment terminating gene transcription, while doxycycline and tamoxifen addition restore gene expression.

Reviewed by eLife