Genetics

Genetic architecture of heart mitochondrial proteome influencing cardiac hypertrophy

1. Karthickeyan Chella Krishnan
2. Elie-Julien El Hachem
3. Mark P Keller
4. Sanjeet G Patel
5. Luke Carroll
6. Alexis Diaz Vegas
7. Isabela Gerdes Gyuricza
8. Christine Light
9. Yang Cao
10. Calvin Pan
11. Karolina Elżbieta Kaczor-Urbanowicz
12. Varun Shravah
13. Diana Anum
14. Matteo Pellegrini
15. Chi Fung Lee
16. Marcus M Seldin
17. Nadia A Rosenthal
18. Gary A Churchill
19. Alan D Attie
20. Benjamin Parker
21. David E James
22. Aldons J Lusis

• Curated by eLife

  eLife assessment

  This paper demonstrates the genetic architecture of heart mitochondrial proteome that influences cardiac hypertrophy, using a panel of inbred mouse strains called the Hybrid Mouse Diversity Panel (HMDP). The HDM panel is a very powerful tool to study the genetic basis of various physiological and pathological processes in mice. The authors have used this panel extensively before, and in this paper, they extend their proteomic studies to demonstrate the genetic basis of cardiac hypertrophy. The studies will allow us to better understand the genetics of hypertrophic cardiomyopathy.

Reviewed by eLife

Rapid and precise genome engineering in a naturally short-lived vertebrate

1. Claire N Bedbrook
2. Ravi D Nath
3. Rahul Nagvekar
4. Karl Deisseroth
5. Anne Brunet

• Curated by eLife

  **eLife assessment
  **
  Within this paper, the authors describe a rapid and easy-to-implement CRISPR/Cas9-mediated knock-in approach to precisely insert large transgenes in the African turquoise killifish. The established method will be instrumental for many researchers working with unusual model species, and, in particular, will expand the killifish community toolbox. It will revolutionize the field and bring the killifish, an emerging animal model in aging biology and disease modeling in vertebrates, into the spotlight even more.

Reviewed by eLife

Cell type–specific interpretation of noncoding variants using deep learning–based methods

1. Maria Sindeeva
2. Nikolay Chekanov
3. Manvel Avetisian
4. Tatiana I Shashkova
5. Nikita Baranov
6. Elian Malkin
7. Alexander Lapin
8. Olga Kardymon
9. Veniamin Fishman

Reviewed by GigaScience

Pooled genome-wide CRISPR activation screening for rapamycin resistance genes in Drosophila cells

1. Baolong Xia
2. Raghuvir Viswanatha
3. Yanhui Hu
4. Stephanie E Mohr
5. Norbert Perrimon

• Curated by eLife

  eLife assessment

  This valuable manuscript presents resources for genome-wide genetic perturbation in the fruitfly, Drosophila. The evidence for the usefulness is solid, with the authors demonstrating that they can identify novel genes that affect an important pathway, the mTOR pathway, which plays key roles in cell proliferation and cell death. The genetic resources are significant for their availability to colleagues in the Drosophila community seeking to to identify genes with important cellular functions.

Reviewed by eLife

Dynamic regulation of inter-organelle communication by ubiquitylation controls skeletal muscle development and disease onset

1. Arian Mansur
2. Remi Joseph
3. Euri S Kim
4. Pierre M Jean-Beltran
5. Namrata D Udeshi
6. Cadence Pearce
7. Hanjie Jiang
8. Reina Iwase
9. Miroslav P Milev
10. Hashem A Almousa
11. Elyshia McNamara
12. Jeffrey Widrick
13. Claudio Perez
14. Gianina Ravenscroft
15. Michael Sacher
16. Philip A Cole
17. Steven A Carr
18. Vandana A Gupta

• Curated by eLife

  eLife assessment

  This important work utilizes a model organism, zebrafish, to explore changes to the proteome and the role of KLHL40, a component of the ubiquitin-proteasome system, in the development of skeletal muscle disease. Using mass spectrometry, the authors demonstrate a major and selective role for proteome remodeling in development. They identify a specific role for KLHL40 deletion in regulating the expression of Sar1 - a key component of biosynthetic secretion, where the resulting elevated levels of Sar1 expression potentially lead to collagen secretion defects in the disease state. The findings are incomplete as further experimental characterization of the overall morphological changes and secretion defects, in particular ones derived from the deregulation of Sar1 levels, is required.

Reviewed by eLife

Her6 and Prox1 are novel regulators of photoreceptor regeneration in the zebrafish retina

1. Kellie Veen
2. Aaron Krylov
3. Shuguang Yu
4. Jie He
5. Patrick Boyd
6. David R. Hyde
7. Theo Mantamadiotis
8. Louise Y Cheng
9. Patricia R Jusuf

Reviewed by Review Commons

Allele-specific gene-editing approach for vision loss restoration in RHO-associated retinitis pigmentosa

1. Xiaozhen Liu
2. Jing Qiao
3. Ruixuan Jia
4. Fan Zhang
5. Xiang Meng
6. Yang Li
7. Liping Yang

• Curated by eLife

  **eLife assessment
  **
  This work provides a valuable allele-specific gene editing therapeutic approach to selectively target the human RHO-T17M mutation, one of the most frequent genetic causes of autosomal dominant retinitis pigmentosa. However, the current data are incomplete. Further validation of gene editing efficiency in rods at cellular level in vivo and use of Rho-T17M mice will strengthen the conclusion.

Reviewed by eLife

Impaired bone strength and bone microstructure in a novel early-onset osteoporotic rat model with a clinically relevant PLS3 mutation

1. Jing Hu
2. Bingna Zhou
3. Xiaoyun Lin
4. Qian Zhang
5. Feifei Guan
6. Lei Sun
7. Jiayi Liu
8. Ou Wang
9. Yan Jiang
10. Wei-bo Xia
11. Xiaoping Xing
12. Mei Li

• Curated by eLife

  eLife assessment

  The findings in this study are important as they establish a rat model of a classic form of early-onset osteoporosis and demonstrate that osteoporosis medications are effective in the model. The evidence supporting the authors' claims is compelling.

Reviewed by eLife

Quantitative trait and transcriptome analysis of genetic complexity underpinning cardiac interatrial septation in mice using an advanced intercross line

1. Mahdi Moradi Marjaneh
2. Edwin P Kirk
3. Ralph Patrick
4. Dimuthu Alankarage
5. David T Humphreys
6. Gonzalo Del Monte-Nieto
7. Paola Cornejo-Paramo
8. Vaibhao Janbandhu
9. Tram B Doan
10. Sally L Dunwoodie
11. Emily S Wong
12. Chris Moran
13. Ian CA Martin
14. Peter C Thomson
15. Richard P Harvey

• Curated by eLife

  eLife assessment

  This important study of mouse genetic variation in atrial septum formation, a trait correlated with the patent foramen ovale (PFO) cardiac defect, provides convincing evidence for 37 quantitative trait loci (QTL) affecting this trait, combining genetic mapping with transcriptome analysis to zero in on relevant pathways and candidate genes within the QTL, and validating the role of one gene in tissue culture. The paper provides an important resource for hypothesis generation and future studies, which could lead to novel diagnostic or therapeutic approaches that target atrial septal defects in common congenital heart disease.

Reviewed by eLife

Precision RNAi using synthetic shRNAmir target sites

1. Thomas Hoffmann
2. Alexandra Hörmann
3. Maja Corcokovic
4. Jakub Zmajkovic
5. Matthias Hinterndorfer
6. Jasko Salkanovic
7. Fiona Spreitzer
8. Anna Köferle
9. Katrin Gitschtaler
10. Alexandra Popa
11. Sarah Oberndorfer
12. Florian Andersch
13. Markus Schaefer
14. Michaela Fellner
15. Nicole Budano
16. Jan G Ruppert
17. Paolo Chetta
18. Melanie Wurm
19. Johannes Zuber
20. Ralph A Neumüller

• Curated by eLife

  eLife assessment

  This manuscript describes a valuable method to study the mechanism of action of essential genes and novel putative drug targets. Evidence for the effectiveness of the system, which is based on engineering pre-validated targets for RNA-mediated knockdown into genes of interest, is compelling, and the method should find use as an orthogonal method for generating gene specific knockdowns.

Reviewed by eLife