Developmental Biology

External signals regulate continuous transcriptional states in hematopoietic stem cells

1. Eva M Fast
2. Audrey Sporrij
3. Margot Manning
4. Edroaldo Lummertz Rocha
5. Song Yang
6. Yi Zhou
7. Jimin Guo
8. Ninib Baryawno
9. Nikolaos Barkas
10. David Scadden
11. Fernando Camargo
12. Leonard I Zon

• Curated by eLife

  Evaluation Summary:

  This is an interesting resource paper, describing the response of hematopoietic stem and progenitor cells to a few drugs commonly used to stress hematopoiesis. Transcriptomic analyses reveal interesting patterns, and the authors use ATAC-seq to investigate whether stem and progenitor subpopulations may be primed to respond in specific ways based on their chromatin accessibility. This turns out not to be the case when directly responsive genes are analyzed, and rather differences can be found in the promoter accessibility of genes further downstream.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Adult stem cells and niche cells segregate gradually from common precursors that build the adult Drosophila ovary during pupal development

1. Amy Reilein
2. Helen V Kogan
3. Rachel Misner
4. Karen Sophia Park
5. Daniel Kalderon

• Curated by eLife

  Evaluation Summary:

  This manuscript describes a very extensive set of experiments charting the origin and fate of various cell populations in the Drosophila ovary that is a powerful system to explore interactions between adult stem cells and their niches. The authors put forward a new view of how different cell types acquire their fates during development. This is a more nuanced view than extant models, involving common progenitors from which different cell fates (stem cell, progeny and niche cells) arise gradually and relying on spatiotemporal cues.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

In vitro endoderm emergence and self-organisation in the absence of extraembryonic tissues and embryonic architecture

1. Stefano Vianello
2. Matthias P. Lutolf

Reviewed by Review Commons

Delilah, prospero, and D-Pax2 constitute a gene regulatory network essential for the development of functional proprioceptors

1. Adel Avetisyan
2. Yael Glatt
3. Maya Cohen
4. Yael Timerman
5. Nitay Aspis
6. Atalya Nachman
7. Naomi Halachmi
8. Ella Preger-Ben Noon
9. Adi Salzberg

• Curated by eLife

  Evaluation Summary:

  The study is carefully performed and provides compelling evidence for a gene network involved in generating different sensory cell types from a common progenitor, showing how an enhancer can integrate a gene regulatory network and guide a cell-fate decision.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife

Loss of Resf1 reduces the efficiency of embryonic stem cell self-renewal and germline entry

1. Matúš Vojtek
2. Ian Chambers

Reviewed by Review Commons

A zebrafish embryo screen utilizing gastrulation identifies the HTR2C inhibitor pizotifen as a suppressor of EMT-mediated metastasis

1. Joji Nakayama
2. Lora Tan
3. Yan Li
4. Boon Cher Goh
5. Shu Wang
6. Hideki Makinoshima
7. Zhiyuan Gong

• Curated by eLife

  Evaluation Summary:

  This manuscript develops a novel approach using the zebrafish to identify suitable drugs against the spread of cancer. With some stronger support of the methodology and conclusions, it will be of interest to cancer biologists, developmental biologists, and pharmacologists.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

SLC1A5 provides glutamine and asparagine necessary for bone development in mice

1. Deepika Sharma
2. Yilin Yu
3. Leyao Shen
4. Guo-Fang Zhang
5. Courtney M Karner

• Curated by eLife

  Evaluation Summary:

  The authors report that intracellular transport of glutamine and asparagine is critical for osteoblast anabolism. The authors use a variety of in vivo and in vitro assays for the testing of their working hypothesis. The paper expands and deepens our knowledge of the role of cellular metabolism in osteoblast function and bone development.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Cardiac pathologies in mouse loss of imprinting models are due to misexpression of H19 long noncoding RNA

1. Ki-Sun Park
2. Beenish Rahat
3. Hyung Chul Lee
4. Zu-Xi Yu
5. Jacob Noeker
6. Apratim Mitra
7. Connor M Kean
8. Russell H Knutsen
9. Danielle Springer
10. Claudia M Gebert
11. Beth A Kozel
12. Karl Pfeifer

• Curated by eLife

  Evaluation Summary:

  This manuscript models a particular class of genetic lesions observed in the imprinting disorder and overgrowth syndrome, Beckwith-Wiedemann syndrome (BWS), using a highly tractable mouse model. Because more than one gene is abnormally expressed in BWS that is caused by loss of imprinting of H19 and IGF2, the authors vary the expression of both genes to investigate the source of the cardiovascular phenotypes and are able to ascribe independent heart phenotypes resulting from IGF2 overexpression and H19 loss of expression.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

Transcriptomic and genetic analyses identify the Krüppel-like factor dar1 as a master regulator of tube-shaped long tendon development

1. Laurichesse Quentin
2. Moucaud Blandine
3. Jagla Krzysztof
4. Soler Cédric

• Curated by eLife

  Evaluation Summary:

  The paper is of interest for those studying tendon development. Starting from a transcriptomic analysis of Drosophila leg tendon cells it identifies a transcription factor, Dar1, required for normal tendon morphogenesis.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

Single-cell RNA sequencing of the Strongylocentrotus purpuratus larva reveals the blueprint of major cell types and nervous system of a non-chordate deuterostome

1. Periklis Paganos
2. Danila Voronov
3. Jacob M Musser
4. Detlev Arendt
5. Maria Ina Arnone

• Curated by eLife

  Evaluation Summary:

  This paper will be of great interest to scientists within the fields of developmental biology and evolution, as well as to researchers that generally use the sea urchin as a model system or those employing single-cell mRNA-sequencing technology. The work provides a comprehensive analysis of the cell state specification of a whole deuterostome organism and proof of principle of the use of single-cell sequencing to identity deep homologies of cell type.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1, #2, and #3 agreed to share their names with the authors.)

Reviewed by eLife