Developmental Biology

Zbtb14 regulates monocyte and macrophage development through inhibiting pu.1 expression in zebrafish

1. Yun Deng
2. Haihong Wang
3. Xiaohui Liu
4. Hao Yuan
5. Jin Xu
6. Hugues de Thé
7. Jun Zhou
8. Jun Zhu

• Curated by eLife

  Evaluation Summary:

  This manuscript by Deng et al., is a valuable evaluation of zbtb14 and its role in normal myelopoiesis. The authors provided convincing data supporting the role played by zbtb14 in monocyte and macrophage development and its regulation involving the modulation of PU.1 expression. The finding that a mutation in ZBTB14 exists in AML patients also implies how important this gene product is in normal human myelopoiesis.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Fibroblast-derived Hgf controls recruitment and expansion of muscle during morphogenesis of the mammalian diaphragm

1. Elizabeth M Sefton
2. Mirialys Gallardo
3. Claire E Tobin
4. Brittany C Collins
5. Mary P Colasanto
6. Allyson J Merrell
7. Gabrielle Kardon

• Curated by eLife

  Evaluation Summary:

  Sefton et al. analyze how fibroblast-derived HGF integrates muscle and nerve development during morphogenesis of the mammalian diaphragm. The new findings are based on in-depth analyses of the development of the diaphragm muscle, and the role of Met and HGF in the process. The work is relevant for the understanding of muscle development, and congenital disease (hernia).

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

Reviewed by eLife

Single‐cell analysis of lymphatic endothelial cell fate specification and differentiation during zebrafish development

1. Lin Grimm
2. Elizabeth Mason
3. Hujun Yu
4. Stefanie Dudczig
5. Virginia Panara
6. Tyrone Chen
7. Neil I Bower
8. Scott Paterson
9. Maria Rondon Galeano
10. Sakurako Kobayashi
11. Anne Senabouth
12. Anne K Lagendijk
13. Joseph Powell
14. Kelly A Smith
15. Kazuhide S Okuda
16. Katarzyna Koltowska
17. Benjamin M Hogan

Reviewed by Review Commons

New functional vessels form after spinal cord injury in zebrafish

1. Ana Ribeiro
2. Mariana Rebocho da Costa
3. Carmen de Sena-Tomás
4. Elsa Charas Rodrigues
5. Raquel Quitéria
6. Tiago Maçarico
7. Susana Constantino Rosa Santos
8. Leonor Saúde

Reviewed by preLights, Review Commons

Airway basal cells show regionally distinct potential to undergo metaplastic differentiation

1. Yizhuo Zhou
2. Ying Yang
3. Lihao Guo
4. Jun Qian
5. Jian Ge
6. Debora Sinner
7. Hongxu Ding
8. Andrea Califano
9. Wellington V Cardoso

• Curated by eLife

  Evaluation Summary:

  Whether airway basal cells exhibit extensive cell state heterogeneity and whether this is relevant for their function has been unclear. This study provides important evidence that such heterogeneity exists and may dictate airway basal cell function in a spatially restricted manner.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife

Glycolytic flux-signaling controls mouse embryo mesoderm development

1. Hidenobu Miyazawa
2. Marteinn T Snaebjornsson
3. Nicole Prior
4. Eleni Kafkia
5. Henrik M Hammarén
6. Nobuko Tsuchida-Straeten
7. Kiran R Patil
8. Martin Beck
9. Alexander Aulehla

Reviewed by Review Commons

Single-cell transcriptomics identifies Keap1-Nrf2 regulated collective invasion in a Drosophila tumor model

1. Deeptiman Chatterjee
2. Caique Almeida Machado Costa
3. Xian-Feng Wang
4. Allison Jevitt
5. Yi-Chun Huang
6. Wu-Min Deng

• Curated by eLife

  Evaluation Summary:

  Drosophila ovarian follicle cells have been utilized as a model system to study organogenesis and epithelial tumorigenesis. The analysis of single-cell transcriptomes of follicle cells now shows that transcriptionally distinct cell populations emerge shortly after induction of loss of polarity. Strengths of the work include the use of advanced single cell omics and imaging analyses to identify cell types and factors playing a role the disruption of polarity and the implications of this work for epithelial cancers. The authors' claims are generally well supported by the data and analyses. Weaknesses include the lack of high magnification images and need to clarify motivation for the study and highlight the biology rather than technical advances in the results section. Overall, this work is viewed as an important contribution to cell biologists who work on the epithelial morphogenesis or tumorigenesis.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1, Reviewer #2 and Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife

Specification of the endocrine primordia controlling insect moulting and metamorphosis by the JAK/STAT signalling pathway

1. Mar García-Ferrés
2. Carlos Sánchez-Higueras
3. Jose Manuel Espinosa-Vázquez
4. James C-G Hombría

Reviewed by Review Commons

Germline/soma distinction in Drosophila embryos requires regulators of zygotic genome activation

1. Megan M Colonnetta
2. Paul Schedl
3. Girish Deshpande

• Curated by eLife

  Evaluation Summary:

  The early differentiation of germ cells, those that will form egg and sperm, is a critical and nearly universal step in animal development. This paper reveals new layers of molecular and cellular regulation that control this process in the fly, and as such be of broad interest to cell and developmental biologists, especially those interested in critical cell fate decisions. The paper contains a wealth of experimental data demonstrating that processes generally thought to be restricted to somatic cells alter the differentiation of germ cells, but provides only limited functional interpretation of the observed phenotypes.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Metamorphosis of memory circuits in Drosophila reveals a strategy for evolving a larval brain

1. James W Truman
2. Jacquelyn Price
3. Rosa L Miyares
4. Tzumin Lee

• Curated by eLife

  Evaluation Summary:

  The complete metamorphosis of the higher insects is one of the most fascinating and complex processes in nature: The discrepancy in form and function between larvae, pupa, and adult insects is breathtaking, begging the question of how these forms and functions can so seamlessly follow each other. For the highest-order brain centre of the insects, the mushroom body, the authors provide a masterpiece analysis of this process at the cellular level. Given the breadth and depth of the data that the authors present, the current study will serve as a reference for the field of developmental neuroscience for many years to come; this study is eagerly awaited in the field. Perhaps ever more importantly, the insights into the relationship between evolutionary development and individual development at the cellular level might have a profound and lasting conceptual impact on life and natural sciences.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife