Developmental Biology

Randomly incorporated genomic N6 ‐methyldeoxyadenosine delays zygotic transcription initiation in a cnidarian

1. Febrimarsa
2. Sebastian G Gornik
3. Sofia N Barreira
4. Miguel Salinas‐Saavedra
5. Christine E Schnitzler
6. Andreas D Baxevanis
7. Uri Frank

Reviewed by Review Commons

A nanobody toolbox to investigate localisation and dynamics of Drosophila titins and other key sarcomeric proteins

1. Vincent Loreau
2. Renate Rees
3. Eunice HoYee Chan
4. Waltraud Taxer
5. Kathrin Gregor
6. Bianka Mußil
7. Christophe Pitaval
8. Nuno Miguel Luis
9. Pierre Mangeol
10. Frank Schnorrer
11. Dirk Görlich

• Curated by eLife

  eLife assessment

  The work describes the generation of novel reagents, nanobodies, which are single molecule antibodies from alpacas, which the authors raised against specific domains of two giant fly muscle proteins called Sallimus and Projectin. These nanobodies, combined with the so-called DNA-Paint approach, enabled the authors to reach an unprecedented spatial resolution and define the position of those domains. Thereby, the authors could propose a model for the organization and extent of those proteins along muscle sarcomeres.

Reviewed by eLife

Cellular reprogramming with ATOH1, GFI1, and POU4F3 implicate epigenetic changes and cell-cell signaling as obstacles to hair cell regeneration in mature mammals

1. Amrita A Iyer
2. Ishwar Hosamani
3. John D Nguyen
4. Tiantian Cai
5. Sunita Singh
6. Melissa M McGovern
7. Lisa Beyer
8. Hongyuan Zhang
9. Hsin-I Jen
10. Rizwan Yousaf
11. Onur Birol
12. Jenny J Sun
13. Russell S Ray
14. Yehoash Raphael
15. Neil Segil
16. Andrew K Groves

• Curated by eLife

  eLife assessment

  Overall, this is an interesting paper that explains molecular underpinnings of hair cell reprogramming. This paper could have significant implications for our understanding of how different cellular programs can dictate phenotypic outcomes such as hearing.

Reviewed by eLife

A conserved function of Human DLC3 and Drosophila Cv-c in testis development

1. Sol Sotillos
2. Isabel von der Decken
3. Ivan Domenech Mercadé
4. Sriraksha Srinivasan
5. Dmytro Sirokha
6. Ludmila Livshits
7. Stefano Vanni
8. Serge Nef
9. Anna Biason-Lauber
10. Daniel Rodríguez Gutiérrez
11. James Castelli-Gair Hombría

Reviewed by Review Commons

The histone chaperone NASP maintains H3-H4 reservoirs in the early Drosophila embryo

1. Reyhaneh Tirgar
2. Jonathan P. Davies
3. Lars Plate
4. Jared T. Nordman

Reviewed by Review Commons

Characterization of an eye field-like state during optic vesicle organoid development

1. Liusaidh J. Owen
2. Jacqueline Rainger
3. Hemant Bengani
4. Fiona Kilanowski
5. David R. FitzPatrick
6. Andrew S. Papanastasiou

Reviewed by Review Commons

Cell cycle and temporal transcription factors regulate proliferation and neuronal diversity of dedifferentiation-derived neural stem cells

1. Kellie Veen
2. Francesca Froldi
3. Qian Dong
4. Edel Alvarez-Ochoa
5. Phuong-Khanh Nguyen
6. Kieran F Harvey
7. John P D McMullen
8. Owen Marshall
9. Patricia R Jusuf
10. Louise Y Cheng

Reviewed by Review Commons

Geometric control of myosin II orientation during axis elongation

1. Matthew F Lefebvre
2. Nikolas H Claussen
3. Noah P Mitchell
4. Hannah J Gustafson
5. Sebastian J Streichan

• Curated by eLife

  eLife assessment

  This paper should be of broad interest to developmental biologists who seek to understand spatiotemporal control of myosin-based force generation during tissue morphogenesis during early development. The central conclusions are well-grounded in rigorous quantitative data analysis and modeling. The results challenge current views of how gene expression patterns control myosin II anisotropies and provide new testable hypotheses on the role and importance of tissue geometry.

Reviewed by eLife

Plap-1 lineage tracing and single-cell transcriptomics reveal cellular dynamics in the periodontal ligament

1. Tomoaki Iwayama
2. Mizuho Iwashita
3. Kazuya Miyashita
4. Hiromi Sakashita
5. Shuji Matsumoto
6. Kiwako Tomita
7. Phan Bhongsatiern
8. Tomomi Kitayama
9. Kentaro Ikegami
10. Takashi Shimbo
11. Katsuto Tamai
12. Masanori A. Murayama
13. Shuhei Ogawa
14. Yoichiro Iwakura
15. Satoru Yamada
16. Lorin E. Olson
17. Masahide Takedachi
18. Shinya Murakami

Reviewed by Review Commons

Human DUX4 and mouse Dux interact with STAT1 and broadly inhibit interferon-stimulated gene induction

1. Amy E Spens
2. Nicholas A Sutliff
3. Sean R Bennett
4. Amy E Campbell
5. Stephen J Tapscott

• Curated by eLife

  eLife assessment

  The transcription factor DUX4 is emerging as a key molecule in early mammalian development and in diverse pathologies including muscular dystrophy and solid tumors. While DUX4 has been linked to immune evasion, the mechanisms have not been delineated. In this study, the authors demonstrate that DUX4 functions as a negative regulator of interferon signaling by inhibiting STAT1, thereby suppressing interferon-stimulated gene induction. These studies provide a critical mechanistic link between DUX4 expression and the modulation of immune signaling pathways.

Reviewed by eLife