Cell Biology

The Nesprin-1/-2 ortholog ANC-1 regulates organelle positioning in C. elegans independently from its KASH or actin-binding domains

1. Hongyan Hao
2. Shilpi Kalra
3. Laura E Jameson
4. Leslie A Guerrero
5. Natalie E Cain
6. Jessica Bolivar
7. Daniel A Starr

Reviewed by eLife

High-content imaging-based pooled CRISPR screens in mammalian cells

1. Xiaowei Yan
2. Nico Stuurman
3. Susana A. Ribeiro
4. Marvin E. Tanenbaum
5. Max A. Horlbeck
6. Christina R. Liem
7. Marco Jost
8. Jonathan S. Weissman
9. Ronald D. Vale

Reviewed by Review Commons, preLights

Quantitative live-cell imaging and computational modeling shed new light on endogenous WNT/CTNNB1 signaling dynamics

1. Saskia MA de Man
2. Gooitzen Zwanenburg
3. Tanne van der Wal
4. Mark A Hink
5. Renée van Amerongen

• Curated by eLife

  Evaluation Summary:

  Wnt signaling plays critical roles in cell fate determination in essentially every tissue in all animals, regulates tissue homeostasis in many adult tissues, and is inappropriately activated in many human cancers. It has been the focus of research for decades, and we have an outline of signal transduction. Remarkably, some of the key questions of Wnt signaling remain controversial. Central among these are questions about the nature of the negative regulatory destruction complex, its mechanism of action and how it is turned down by Wnt signaling. Here Saskia and colleagues take a novel and very exciting approach to these questions, combining innovative quantitative live-cell imaging and computational modelling.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife

Organization of DNA Replication Origin Firing in Xenopus Egg Extracts: The Role of Intra-S Checkpoint

1. Diletta Ciardo
2. Olivier Haccard
3. Hemalatha Narassimprakash
4. Jean-Michel Arbona
5. Olivier Hyrien
6. Benjamin Audit
7. Kathrin Marheineke
8. Arach Goldar

Reviewed by eLife

CDK2 activity determines the timing of cell-cycle commitment and sequential DNA replication

1. Sungsoo Kim
2. Alessandra Leong
3. Chellam Nayar
4. Minah Kim
5. Hee Won Yang

Reviewed by eLife

Increasing heart vascularisation after myocardial infarction using brain natriuretic peptide stimulation of endothelial and WT1+ epicardial cells

1. Na Li
2. Stephanie Rignault-Clerc
3. Christelle Bielmann
4. Anne-Charlotte Bon-Mathier
5. Tamara Déglise
6. Alexia Carboni
7. Mégane Ducrest
8. Nathalie Rosenblatt-Velin

Reviewed by eLife

Pregnancy-associated plasma protein-aa regulates endoplasmic reticulum–mitochondria associations

1. Mroj Alassaf
2. Mary C Halloran

Reviewed by eLife

Compartment-specific opioid receptor signaling is selectively modulated by different dynorphin peptides

1. Jennifer M Kunselman
2. Achla Gupta
3. Ivone Gomes
4. Lakshmi A Devi
5. Manojkumar A Puthenveedu

Reviewed by eLife

Extensive ribosome and RF2 rearrangements during translation termination

1. Egor Svidritskiy
2. Gabriel Demo
3. Anna B Loveland
4. Chen Xu
5. Andrei A Korostelev

Reviewed by eLife

The ribosomal RNA m5C methyltransferase NSUN-1 modulates healthspan and oogenesis in Caenorhabditis elegans

1. Clemens Heissenberger
2. Jarod A Rollins
3. Teresa L Krammer
4. Fabian Nagelreiter
5. Isabella Stocker
6. Ludivine Wacheul
7. Anton Shpylovyi
8. Koray Tav
9. Santina Snow
10. Johannes Grillari
11. Aric N Rogers
12. Denis L J Lafontaine
13. Markus Schosserer

Reviewed by eLife