Cell Biology

Mir155 regulates osteogenesis and bone mass phenotype via targeting S1pr1 gene

1. Zhichao Zheng
2. Lihong Wu
3. Zhicong Li
4. Ruoshu Tang
5. Hongtao Li
6. Yinyin Huang
7. Tianqi Wang
8. Shaofen Xu
9. Haoyu Cheng
10. Zhitong Ye
11. Dong Xiao
12. Xiaolin Lin
13. Gang Wu
14. Richard T Jaspers
15. Janak L Pathak

• Curated by eLife

  Evaluation Summary:

  Caner/cancer metastasis-induced bone loss-mediated fracture is a serious clinical problem. In this regard the authors have done an interesting study to show how miR155 exhibits a catabolic effect on osteogenesis and bone mass phenotype via interaction with the Sphingosine 1-phosphate receptor-1 (S1PR1) gene. The study suggests that inhibition of miR155 could be a potential strategy for bone regeneration and bone defect healing.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife

Centriole growth is limited by the Cdk/Cyclin-dependent phosphorylation of Ana2/STIL

1. Thomas L. Steinacker
2. Siu-Shing Wong
3. Zsofia A. Novak
4. Saroj Saurya
5. Lisa Gartenmann
6. Eline J.H. van Houtum
7. Judith R. Sayers
8. B. Christoffer Lagerholm
9. Jordan W. Raff

Reviewed by Review Commons

Actin network architecture can ensure robust centering or sensitive decentering of the centrosome

1. Shohei Yamamoto
2. Jérémie Gaillard
3. Benoit Vianay
4. Christophe Guerin
5. Magali Orhant‐Prioux
6. Laurent Blanchoin
7. Manuel Théry

Reviewed by Review Commons

Novel ACE2 nanoparticles universally block SARS-CoV-2 variants in the human respiratory tract

1. Cécile Sauvanet
2. Moara Lemos
3. Armel Bezault
4. Borja Rodríguez de Francisco
5. Michael CW Chan
6. Kenrie PY Hui
7. Ka-chun Ng
8. John M Nicholls
9. Niels Volkmann
10. Dorit Hanein

Reviewed by ScreenIT

High-resolution secretory timeline from vesicle formation at the Golgi to fusion at the plasma membrane in S. cerevisiae

1. Robert M Gingras
2. Abigail M Sulpizio
3. Joelle Park
4. Anthony Bretscher

• Curated by eLife

  Evaluation Summary:

  The process of secretory vesicle formation, transport, and fusion in yeast has mainly been characterized through biochemical and genetic means. Only limited information was available about the detailed timeline and order of events. This study fills the gap with a high-resolution temporal analysis, which provides new insights into when key components arrive and depart and how they promote vesicle tethering and fusion. The work is experimentally strong, and improvements to the presentation will ensure that the findings are communicated effectively.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

Allelic gene polymorphisms suspected to diversify the individual early metabolic response upon influenza H3N2 and SARS-CoV-2 infections

1. Birgit Arnholdt-Schmitt
2. Shahid Aziz
3. José Hélio Costa

Reviewed by ScreenIT

CXCR4high megakaryocytes regulate host-defense immunity against bacterial pathogens

1. Jin Wang
2. Jiayi Xie
3. Daosong Wang
4. Xue Han
5. Minqi Chen
6. Guojun Shi
7. Linjia Jiang
8. Meng Zhao

• Curated by eLife

  Evaluation Summary:

  The manuscript studies the heterogeneity of megakaryocytes using single cell RNA-seq and identifies a subpopulation of CXCR4-high megakaryocytes with immune modulatory roles. The authors also perform functional studies which show that this subpopulation of megakaryocytes promotes bacterial phagocytosis by macrophages and neutrophils. This work would be of significant interest to researchers in the fields of immunology and host defense as well as researchers studying hematopoiesis and megakaryocyte biology because it provides new perspectives on megakaryocyte heterogeneity as well as the role of megakaryocytes in host defense and immune function.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

Functional requirements for a Samd14-capping protein complex in stress erythropoiesis

1. Suhita Ray
2. Linda Chee
3. Yichao Zhou
4. Meg A Schaefer
5. Michael J Naldrett
6. Sophie Alvarez
7. Nicholas T Woods
8. Kyle J Hewitt

• Curated by eLife

  Evaluation Summary:

  This is an interesting study describing the role of Samd14-capping protein (CP) complex in stress erythroid signaling. Using orthogonal cellular, biochemical and genetic complementation approaches, the authors provide evidence to establish a previously unrecognized mechanism for Samd14-CP interaction in regulating Kit signaling in erythroid regeneration in response to acute anemia. Findings of this work will be of broad interest to the study of erythropoiesis and cellular signaling.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife

Claudin5 protects the peripheral endothelial barrier in an organ and vessel-type-specific manner

1. Mark Richards
2. Emmanuel Nwadozi
3. Sagnik Pal
4. Pernilla Martinsson
5. Mika Kaakinen
6. Marleen Gloger
7. Elin Sjöberg
8. Katarzyna Koltowska
9. Christer Betsholtz
10. Lauri Eklund
11. Sofia Nordling
12. Lena Claesson-Welsh

• Curated by eLife

  Evaluation Summary:

  This manuscript provides new knowledge of one of the major molecules, claudin5, expected to maintain the integrity of the cell-cell contacts of the blood vessel wall. The results highlight organ and vessel-type specific significance of this mechanism in the regulation of vascular permeability, partially challenging the current view. A combination of in vivo microscopy and genetic mouse models is used to support the key claims in the paper. This manuscript will be of interest to scientists across vascular biology, and especially in the field of vascular permeability regulation.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife

An amphipathic helix in Brl1 is required for nuclear pore complex biogenesis in S. cerevisiae

1. Annemarie Kralt
2. Matthias Wojtynek
3. Jonas S Fischer
4. Arantxa Agote-Aran
5. Roberta Mancini
6. Elisa Dultz
7. Elad Noor
8. Federico Uliana
9. Marianna Tatarek-Nossol
10. Wolfram Antonin
11. Evgeny Onischenko
12. Ohad Medalia
13. Karsten Weis

• Curated by eLife

  Evaluation Summary:

  This work advances our understanding of the nuclear pore complex (NPC) biogenesis pathway by providing much needed additional insight into the function of one of the few NPC assembly factors, Brl1. It thus addresses a long-standing and fundamental question relevant to individuals interested in nuclear transport, nuclear cell biology, and membrane-protein interactions more generally.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife