Bioengineering

Scalable, methanol‐free manufacturing of the SARS‐CoV‐2 receptor‐binding domain in engineered Komagataella phaffii

1. Neil C. Dalvie
2. Andrew M. Biedermann
3. Sergio A. Rodriguez‐Aponte
4. Christopher A. Naranjo
5. Harish D. Rao
6. Meghraj P. Rajurkar
7. Rakesh R. Lothe
8. Umesh S. Shaligram
9. Ryan S. Johnston
10. Laura E. Crowell
11. Seraphin Castelino
12. Mary K. Tracey
13. Charles A. Whittaker
14. J. Christopher Love

Reviewed by ScreenIT

Application of an integrated computational antibody engineering platform to design SARS-CoV-2 neutralizers

1. Saleh Riahi
2. Jae Hyeon Lee
3. Shuai Wei
4. Robert Cost
5. Alessandro Masiero
6. Catherine Prades
7. Reza Olfati-Saber
8. Maria Wendt
9. Anna Park
10. Yu Qiu
11. Yanfeng Zhou

Reviewed by ScreenIT

Multiple sites on SARS‐CoV ‐2 spike protein are susceptible to proteolysis by cathepsins B, K, L, S, and V

1. Keval Bollavaram
2. Tiffanie H. Leeman
3. Maggie W. Lee
4. Akhil Kulkarni
5. Sophia G. Upshaw
6. Jiabei Yang
7. Hannah Song
8. Manu O. Platt

Reviewed by ScreenIT

Multi-modal engineering of Bst DNA polymerase for thermostability in ultra-fast LAMP reactions

1. Inyup Paik
2. Phuoc H. T. Ngo
3. Raghav Shroff
4. Andre C. Maranhao
5. David J.F. Walker
6. Sanchita Bhadra
7. Andrew D. Ellington

Reviewed by ScreenIT

Peptide Antidotes to SARS-CoV-2 (COVID-19)

1. Andre Watson
2. Leonardo Ferreira
3. Peter Hwang
4. Jinbo Xu
5. Robert Stroud

Reviewed by ScreenIT

Massively multiplexed affinity characterization of therapeutic antibodies against SARS-CoV-2 variants

1. Emily Engelhart
2. Randolph Lopez
3. Ryan Emerson
4. Charles Lin
5. Colleen Shikany
6. Daniel Guion
7. Mary Kelley
8. David Younger

Reviewed by ScreenIT

In vitro rapid inactivation of SARS-CoV-2 by visible light photocatalysis using boron-doped bismuth oxybromide

1. Li Ling
2. Tea Carletti
3. Zihang Cheng
4. Ruixuan Wang
5. Yanxiao Ren
6. Paul Westerhoff
7. Chii Shang
8. Alessandro Marcello
9. Shuyu Chen

Reviewed by ScreenIT

An implant for long-term cervical vagus nerve stimulation in mice

1. Ibrahim T. Mughrabi
2. Jordan Hickman
3. Naveen Jayaprakash
4. Eleni S. Papadoyannis
5. Adam Abbas
6. Yao-Chuan Chang
7. Sunhee Lee
8. Timir Datta-Chaudhuri
9. Eric H. Chang
10. Theodoros P. Zanos
11. Robert C. Froemke
12. Cristin Welle
13. Yousef Al-Abed
14. Stavros Zanos

Reviewed by eLife

Host-associated microbe PCR (hamPCR): accessing new biology through convenient measurement of both microbial load and community composition

1. Derek S. Lundberg
2. Pratchaya Pramoj Na Ayutthaya
3. Annett Strauß
4. Gautam Shirsekar
5. Wen-Sui Lo
6. Thomas Lahaye
7. Detlef Weigel

• Curated by eLife

  Evaluation Summary:

  Overall, we agree that this new method is potentially impactful although the full versatility of the approach is currently unclear for several reasons. We appreciated the application of the approach to distinct systems and also the relatively low cost of this technique. The diagrams presented (particularly in Figure 3) nicely convey the steps in the protocol with expected sample outcomes to further facilitate the ability of other researchers to employ hamPCR. Overall, we are very positive about this work, but given that the impact of this paper rests on whether or not the technique is widely adopted, some revisions will lower the barrier to entry for future researchers to adopt this approach.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

Reviewed by eLife

Innervated adrenomedullary microphysiological system to model prenatal nicotine and opioid exposure

1. Jonathan R. Soucy
2. Gabriel Burchett
3. Ryan Brady
4. David T. Breault
5. Abigail N. Koppes
6. Ryan A. Koppes

• Curated by eLife

  Summary: All three reviewers were not convinced that this screening platform has been properly validated vis-à-vis the neurobiology of the adrenal gland, nor that it has a physiologic relevance for the understanding of living processes and organs.

Reviewed by eLife