Biochemistry

Piezo1 as a force-through-membrane sensor in red blood cells

1. George Vaisey
2. Priyam Banerjee
3. Alison J North
4. Christoph A Haselwandter
5. Roderick MacKinnon

• Curated by eLife

  eLife assessment

  This important paper uses advanced imaging approaches to explore how Piezo1 distributes on surface red blood cells. The study provides compelling evidence that this molecule 'reads' the membrane curvature and clear support for the force-through-membrane model of mechanosensation.

Reviewed by eLife

Robust capability of renal tubule fatty acid uptake from apical and basolateral membranes in physiology and disease

1. Ryo Kawakami
2. Hirofumi Hanaoka
3. Ayaka Kanai
4. Hideru Obinata
5. Daisuke Nakano
6. Hidekazu Ikeuchi
7. Miki Matsui
8. Toshiyuki Matsuzaki
9. Rina Tanaka
10. Hiroaki Sunaga
11. Sawako Goto
12. Hiroki Matsui
13. Norimichi Koitabashi
14. Keiko Saegusa
15. Tomoyuki Yokoyama
16. Keiju Hiromura
17. Akira Nishiyama
18. Akihiko Saito
19. Motoko Yanagita
20. Hideki Ishii
21. Masahiko Kurabayashi
22. Tatsuya Iso

• Curated by eLife

  eLife assessment

  This manuscript presents valuable and significant data on how lipids may accumulate in the tubulointerstitial compartment of the diseased kidney, but the work is largely descriptive, using methods that are inadequate for quantification (colorimetric assays versus mass spec), thus rendering data interpretation not very convincing. Therefore, while a major strength is the presentation of innovative ideas, additional experiments would be needed to support the main conclusions.

Reviewed by eLife

An acetylation-mediated chromatin switch governs H3K4 methylation read-write capability

1. Kanishk Jain
2. Matthew R Marunde
3. Jonathan M Burg
4. Susan L Gloor
5. Faith M Joseph
6. Karl F Poncha
7. Zachary B Gillespie
8. Keli L Rodriguez
9. Irina K Popova
10. Nathan W Hall
11. Anup Vaidya
12. Sarah A Howard
13. Hailey F Taylor
14. Laylo Mukhsinova
15. Ugochi C Onuoha
16. Emily F Patteson
17. Spencer W Cooke
18. Bethany C Taylor
19. Ellen N Weinzapfel
20. Marcus A Cheek
21. Matthew J Meiners
22. Geoffrey C Fox
23. Kevin EW Namitz
24. Martis W Cowles
25. Krzysztof Krajewski
26. Zu-Wen Sun
27. Michael S Cosgrove
28. Nicolas L Young
29. Michael-Christopher Keogh
30. Brian D Strahl

• Curated by eLife

  eLife assessment

  This study shows that cis H3 tail acetylation promotes nucleosome accessibility to H3K4 methyl readers and writers such as MLL1. The findings provide a molecular basis for the long-standing connection between H3 acetylation and H3K4 methylation. Additional evidence is required to fully support the conclusions.

Reviewed by eLife

Profiling dynamic RNA–protein interactions using small-molecule-induced RNA editing

1. Kyung W. Seo
2. Ralph E. Kleiner

Reviewed by ASAPbio crowd review

N-terminal domain on dystroglycan enables LARGE1 to extend matriglycan on α-dystroglycan and prevents muscular dystrophy

1. Hidehiko Okuma
2. Jeffrey M Hord
3. Ishita Chandel
4. David Venzke
5. Mary E Anderson
6. Ameya S Walimbe
7. Soumya Joseph
8. Zeita Gastel
9. Yuji Hara
10. Fumiaki Saito
11. Kiichiro Matsumura
12. Kevin P Campbell

• Curated by eLife

  eLife assessment

  The authors show that the amino terminus of dystroglycan is required for the production of full-length matriglycan, and in its absence, a shorter matriglycan is produced that is still capable of binding laminin. alpha-DGN deficient mice have abnormal neuromuscular synapses and reduced lengthening contraction-induced force. Overall, the well-controlled and convincing data mostly support the main conclusions, which will be of interest to scientists in membrane biology, muscle biology, and glycobiology.

Reviewed by eLife

The conserved centrosomin motif, γTuNA, forms a dimer that directly activates microtubule nucleation by the γ-tubulin ring complex (γTuRC)

1. Michael J Rale
2. Brianna Romer
3. Brian P Mahon
4. Sophie M Travis
5. Sabine Petry

• Curated by eLife

  eLife assessment

  This paper is of interest to cell biologists studying the mechanisms and control of microtubule nucleation. In this work, the authors use a novel protocol for the purification of gamma-TuRCs and for the production of gamma-TuNA that enables them to demonstrate a clear activating effect of gamma-TuNA on microtubule nucleation that depends on the dimerization of gamma-TuNA protein chains.

Reviewed by eLife

ATP-competitive and allosteric inhibitors induce differential conformational changes at the autoinhibitory interface of Akt

1. Alexandria L Shaw
2. Matthew AH Parson
3. Linda Truebestein
4. Meredith L Jenkins
5. Thomas A Leonard
6. John E Burke

Reviewed by ASAPbio crowd review

MitoStores: chaperone‐controlled protein granules store mitochondrial precursors in the cytosol

1. Lena Krämer
2. Niko Dalheimer
3. Markus Räschle
4. Zuzana Storchová
5. Jan Pielage
6. Felix Boos
7. Johannes M Herrmann

Reviewed by ASAPbio crowd review

Tuning aromatic contributions by site-specific encoding of fluorinated phenylalanine residues in bacterial and mammalian cells

1. Grace D. Galles
2. Daniel T. Infield
3. Colin J. Clark
4. Marcus L. Hemshorn
5. Shivani Manikandan
6. Frederico Fazan
7. Ali Rasouli
8. Emad Tajkhorshid
9. Jason D. Galpin
10. Richard B. Cooley
11. Ryan A. Mehl
12. Christopher A. Ahern

• Curated by Biophysics Colab

  Endorsement statement (3 October 2022)

  The preprint by Galles et al. reports the generation of pyrrolysine-based aminoacyl-tRNA synthetases capable of incorporating fluorinated phenylalanine non-canonical amino acids into proteins expressed in either bacteria or mammalian cells. For the most extensively characterized synthetases, fluorinated phenylalanine derivatives were successfully incorporated into GFP and two membrane proteins (CFTR and Nav1.5) at expression levels adequate for biochemical studies, suggesting that the approach could be combined with multiple different structural and biophysical techniques. The work provides a valuable tool that will enable the functional role of cation-pi interactions to be interrogated in both soluble and integral membrane proteins.

  (This endorsement by Biophysics Colab refers to version 2 of this preprint, which has been revised in response to peer review of version 1.)

Reviewed by Biophysics Colab

Adiponectin reverses β‐Cell damage and impaired insulin secretion induced by obesity

1. Ana Cláudia Munhoz
2. Julian D. C. Serna
3. Eloisa Aparecida Vilas‐Boas
4. Camille C. Caldeira da Silva
5. Tiago G. Santos
6. Francielle C. Mosele
7. Sergio L. Felisbino
8. Vilma Regina Martins
9. Alicia J. Kowaltowski

Reviewed by ASAPbio crowd review