Molecular Beacons Allow Specific RT-LAMP Detection of B.1.1.7 Variant SARS-CoV-2
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SciScore for 10.1101/2021.03.25.21254356: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Software and Algorithms Sentences Resources We used the Primer Explorer software to design a LAMP primer set (S6970) spanning the S1Δ69-70 deletion such that the deletion falls within the backward loop region of the amplification (Table 1) and then designed a molecular beacon (S6970 B117 MB) to both land inside the loop and span the deletion. Primer Explorersuggested: NoneWe also added locked nucleic acids to increase specificity and strengthen hybridization to allow real time detection at the temperatures used in LAMP. LA…SciScore for 10.1101/2021.03.25.21254356: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Software and Algorithms Sentences Resources We used the Primer Explorer software to design a LAMP primer set (S6970) spanning the S1Δ69-70 deletion such that the deletion falls within the backward loop region of the amplification (Table 1) and then designed a molecular beacon (S6970 B117 MB) to both land inside the loop and span the deletion. Primer Explorersuggested: NoneWe also added locked nucleic acids to increase specificity and strengthen hybridization to allow real time detection at the temperatures used in LAMP. LAMPsuggested: (LAMP, RRID:SCR_001740)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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