Detection and Serotyping of Dengue Virus by Single-Plex Multicolor Real Time RT-qPCR
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Dengue remains one of the most significant tropical diseases, with its geographic range continuing to expand. The etiological agent of the disease is dengue virus, a term that groups four closely related viruses defined as serotypes. The four dengue virus serotypes elicit distinct serological responses, and their replacement or co-circulation in endemic regions can result in major outbreaks. The identification of the circulating serotypes is essential for surveillance and clinical management. Here we describe a novel method for the detection and serotyping of the four dengue virus serotypes using a single plex multicolor real-time RT-qPCR. The method takes advantage of a highly informative dataset that includes a realistic representation of globally circulating strains, allowing the selection of a universal primer pair and four serotype-specific hydrolysis probes. The method has been extensively tested and the results support its use in surveillance studies as well as in clinical settings.