Development and Potential Usefulness of the COVID-19 Ag Respi-Strip Diagnostic Assay in a Pandemic Context

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1. 

   ScreenIT

   Jun 6, 2020

   SciScore for 10.1101/2020.04.24.20077776: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	Duplicate specimen were randomly chosen.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   Development of the COVID-19 Ag Respi-Strip: Analytical performance study: During the development phase, analytical performance was first performed on serial dilution of cultured virus in parallel of tittering the same virus preparation on Vero E6 cells and testing by qRT-PCR.	Vero E6 suggested: RRID:CVCL_XD71)
   Software and Algorithms
   Sentences	Resources
   At the laboratory LHUB-ULB, viral RNA extraction was …

   SciScore for 10.1101/2020.04.24.20077776: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	Duplicate specimen were randomly chosen.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   Development of the COVID-19 Ag Respi-Strip: Analytical performance study: During the development phase, analytical performance was first performed on serial dilution of cultured virus in parallel of tittering the same virus preparation on Vero E6 cells and testing by qRT-PCR.	Vero E6 suggested: RRID:CVCL_XD71)
   Software and Algorithms
   Sentences	Resources
   At the laboratory LHUB-ULB, viral RNA extraction was performed by QIAsymphony DSP Virus/Pathogen Kit (QIAGEN) which extracted 400µl sample eluted in 60µl elution buffer for the Mini Kit and 800µl sample eluted in 110µl buffer for the Midi Kit, or by m2000 mSample Preparation SystemDNA Kit (Abbott) using 1000µl manually lysed sample (700µl sample + 800µl lysis buffer from kit) eluted in 90µl elution buffer.	Abbott suggested: (Abbott, RRID:SCR_010477)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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   Read the original source
2. Version published to 10.3389/fmed.2020.00225

   May 8, 2020
3. 

   Version published to 10.1101/2020.04.24.20077776 on medRxiv

   Apr 29, 2020