A Combination Adjuvant for the Induction of Potent Antiviral Immune Responses for a Recombinant SARS-CoV-2 Protein Vaccine

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1. Version published to 10.3389/fimmu.2021.729189

   Sep 16, 2021
2. 

   Version published to 10.1101/2021.02.18.431484v2 on bioRxiv

   May 3, 2021
3. 

   ScreenIT

   Mar 1, 2021

   SciScore for 10.1101/2021.02.18.431484: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	IACUC: Animals: All animal procedures were approved by the Institutional Animal Care and Use Committees (IACUC) at the University of Michigan and Icahn School of Medicine at Mt. Sinai and were carried out in accordance with these guidelines. 6-8-week-old female C57Bl/6 mice (Charles River Laboratories) were housed in specific pathogen-free conditions.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	Animals: All animal procedures were approved by the Institutional Animal Care and Use Committees (IACUC) at the University of Michigan and Icahn School of Medicine at Mt. Sinai and were carried out in accordance …

   SciScore for 10.1101/2021.02.18.431484: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	IACUC: Animals: All animal procedures were approved by the Institutional Animal Care and Use Committees (IACUC) at the University of Michigan and Icahn School of Medicine at Mt. Sinai and were carried out in accordance with these guidelines. 6-8-week-old female C57Bl/6 mice (Charles River Laboratories) were housed in specific pathogen-free conditions.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	Animals: All animal procedures were approved by the Institutional Animal Care and Use Committees (IACUC) at the University of Michigan and Icahn School of Medicine at Mt. Sinai and were carried out in accordance with these guidelines. 6-8-week-old female C57Bl/6 mice (Charles River Laboratories) were housed in specific pathogen-free conditions.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   Plates were washed three times with PBST (0.05% Tween20), and alkaline phosphatase conjugated secondary antibodies were added (goat-anti-mouse IgG, IgG1, IgG2b, or IgG2c Jackson Immuno Research Laboratories) diluted in PBS/0.1%BSA.	IgG, IgG1 suggested: None
   Cells were permeabilized with 0.1% TritonX100, washed and incubated with anti-SARS-CoV-2-nucleoprotein and anti-SARS-CoV-2-Spike monoclonal antibodies, mixed in 1:1 ratio, for 1.5h at RT.	anti-SARS-CoV-2-nucleoprotein suggested: None anti-SARS-CoV-2-Spike suggested: None
   After another wash, cells were incubated with HRP-conjugated goat-anti-mouse IgG secondary antibody for 1h at RT.	IgG suggested: None
   Anti-mouse SARS-CoV-2-nucleoprotein and anti-mouse SARS-CoV-2-spike antibodies were obtained from the Center for Therapeutic Antibody Development at the Icahn School of Medicine at Mount Sinai.	Anti-mouse SARS-CoV-2-nucleoprotein suggested: None anti-mouse SARS-CoV-2-spike suggested: None
   Experimental Models: Cell Lines
   Sentences	Resources
   Briefly, SeV DI RNA from SeV-infected A549 cells was amplified using a 5’ primer with the T7 promoter and a 3’ primer with the hepatitis delta virus genomic ribozyme site followed by the T7 terminator.	A549 suggested: NCI-DTP Cat# A549, RRID:CVCL_0023)
   HEK293T cells expressing human angiotensin-converting enzyme 2 (293T-hACE2) were obtained from BEI resources and maintained in HEK293T medium: DMEM containing 4 mM L-glutamine, 4500 mg/L L-glucose, 1 mM sodium pyruvate and 1500 mg/L sodium bicarbonate, supplemented with 10% heat inactivated fetal bovine serum as previously described48.	HEK293T suggested: ATCC Cat# ACS-4500, RRID:CVCL_4V93)
   Viruses: WT SARS-CoV-2: SARS-CoV-2 clinical isolate USA-WA1/2020 (BEI resources; NR-52281), was propagated by culture in Vero E6 cells as previously described (ref).	Vero E6 suggested: RRID:CVCL_XD71)
   This DNA/PEI containing media was then distributed equally to 5-T150 flasks (Falcon) containing 293T cells.	293T suggested: NCBI_Iran Cat# C498, RRID:CVCL_0063)
   Pseudovirus microneutralization (MNT) assays: 9×103 293T-hACE2 cells were seeded overnight on white clear bottom 96-well tissue culture plates in HEK293T medium.	293T-hACE2 suggested: None
   Experimental Models: Organisms/Strains
   Sentences	Resources
   Animals: All animal procedures were approved by the Institutional Animal Care and Use Committees (IACUC) at the University of Michigan and Icahn School of Medicine at Mt. Sinai and were carried out in accordance with these guidelines. 6-8-week-old female C57Bl/6 mice (Charles River Laboratories) were housed in specific pathogen-free conditions.	C57Bl/6 suggested: None

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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4. 

   Version published to 10.1101/2021.02.18.431484v1 on bioRxiv

   Feb 18, 2021