Development and Validation of a Rapid, Single-Step Reverse Transcriptase Loop-Mediated Isothermal Amplification (RT-LAMP) System Potentially to Be Used for Reliable and High-Throughput Screening of COVID-19
This article has been Reviewed by the following groups
Listed in
- Evaluated articles (ScreenIT)
Abstract
No abstract available
Article activity feed
-
-
SciScore for 10.1101/2020.03.15.20036376: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: The ethics committees of both centers approved the study. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources Therefore, ORF1ab and N sequences of SARS-Cov-2, its close related coronavirus species (HCoV-NL63, HCoV-OC43, HCoV-229E and HCoV-HKU1), and other viral species, namely Adenovirus, Respiratory syncytial virus A, Human parainfluenza 2 virus HCoV-NL63suggested: RRID:CVCL_RW88)Moreover, HeLa cells (TechStar Co. Ltd, Jiangsu, China) and genomic DNA of clinically prominent bacteria or fungal … SciScore for 10.1101/2020.03.15.20036376: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: The ethics committees of both centers approved the study. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources Therefore, ORF1ab and N sequences of SARS-Cov-2, its close related coronavirus species (HCoV-NL63, HCoV-OC43, HCoV-229E and HCoV-HKU1), and other viral species, namely Adenovirus, Respiratory syncytial virus A, Human parainfluenza 2 virus HCoV-NL63suggested: RRID:CVCL_RW88)Moreover, HeLa cells (TechStar Co. Ltd, Jiangsu, China) and genomic DNA of clinically prominent bacteria or fungal species, including Staphylococcus aureus, Mycobacterium tuberculosis HeLasuggested: CLS Cat# 300194/p772_HeLa, RRID:CVCL_0030)Software and Algorithms Sentences Resources Designed primers were subjected to BLAST (https://blast.ncbi.nlm.nih.gov/Blast.cgi) and the specific candidates were used for analytical sensitivity and specificity testing (Table 1). BLASTsuggested: (BLASTX, RRID:SCR_001653)https://blast.ncbi.nlm.nih.gov/Blast.cgisuggested: (TBLASTX, RRID:SCR_011823)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:These two limitations will be the subject of future investigation. Moreover, we will try to use simple and fast nucleic acid extraction procedures (7) that only uses heat that will further decrease the turn-around-time.
Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
-
-