Susceptibility of Wild Canids to SARS-CoV-2
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SciScore for 10.1101/2022.01.27.478082: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics Field Sample Permit: Animal work was approved by CSU and National Wildlife Research Center Institutional Animal Care and Use Committees.
IACUC: Animal work was approved by CSU and National Wildlife Research Center Institutional Animal Care and Use Committees.Sex as a biological variable Animals: Captive reared, juvenile (3-5-month-old), mixed sex red fox (3 female, 3 male) and coyotes (3 female, 1 male) were evaluated for susceptibility to SARS-CoV-2. Randomization not detected. Blinding not detected. Power Analysis not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources Virus: SARS-COV-2 strain WA1/2020WY96 obtained from BEI Resources was … SciScore for 10.1101/2022.01.27.478082: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics Field Sample Permit: Animal work was approved by CSU and National Wildlife Research Center Institutional Animal Care and Use Committees.
IACUC: Animal work was approved by CSU and National Wildlife Research Center Institutional Animal Care and Use Committees.Sex as a biological variable Animals: Captive reared, juvenile (3-5-month-old), mixed sex red fox (3 female, 3 male) and coyotes (3 female, 1 male) were evaluated for susceptibility to SARS-CoV-2. Randomization not detected. Blinding not detected. Power Analysis not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources Virus: SARS-COV-2 strain WA1/2020WY96 obtained from BEI Resources was passaged twice in Vero E6 cells, and stock virus harvested in Dulbecco’s Modified Eagle Medium (DMEM) with 5% fetal bovine serum and antibiotics was frozen at -80°C. Vero E6suggested: NoneVirus back-titration on Vero cells was then immediately performed; each animal was confirmed to have received between 5.1-6.0 log10 pfu of SARS-CoV-2. Sampling: Animals were lightly anesthetized using the aforementioned protocol for all sample collections. Verosuggested: NoneExperimental Models: Organisms/Strains Sentences Resources Virus: SARS-COV-2 strain WA1/2020WY96 obtained from BEI Resources was passaged twice in Vero E6 cells, and stock virus harvested in Dulbecco’s Modified Eagle Medium (DMEM) with 5% fetal bovine serum and antibiotics was frozen at -80°C. WA1/2020WY96suggested: NoneResults from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:While small sample sizes and direct intranasal inoculation with a high dose of virus are limitations of experimental challenge studies like this one, these studies do provide important information that can inform continued surveillance and control efforts. The animals found to be susceptible to natural infection with SARS-CoV-2 have reflected results from experimental challenge studies, so it is reasonable to assume that our results can be extrapolated. Therefore, special attention should be paid to red fox when considering wildlife species that may serve as reservoir hosts for SARS-CoV-2. We demonstrated that SARS-CoV-2 infected red fox shed infectious virus for multiple days in both oral and nasal secretions, which suggests that red fox could contribute to onward transmission of the virus. As red fox commonly consume, via hunting or scavenging, other species that are susceptible to infection with SARS-CoV-2, including rodents and white-tailed deer (20), predator-prey interactions and scavenging may serve as avenues for inter-species transmission. Should wildlife species such as red fox become established maintenance hosts of SARS-CoV-2, consequences could include impacts on animal health, development of novel viral variants, and spillback into the human population. Caution should be taken when interacting with susceptible wildlife species in order to prevent transmission events. To best curtail the COVID-19 pandemic, we should understand all that may contribute to the conti...
Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
Results from scite Reference Check: We found no unreliable references.
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