Sensitive Detection of SARS-CoV-2–Specific Antibodies in Dried Blood Spot Samples
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Abstract
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SciScore for 10.1101/2020.07.01.20144295: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding Laboratory analysis was blinded to PCR status and SARS-CoV-2-specific-antibody results were reported as positive, negative or equivocal. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Antibodies Sentences Resources Mouse monoclonal anti-human HRP-conjugated antibodies (anti-IgG clone R-10 1:8000, anti-IgA MG4.156 1:4000 and anti-IgM AF6 1:2000; clones were generated at the University of Birmingham, available from Abingdon Health, UK) were diluted in 0.1% PBS-T. anti-human HRP-conjugated antibodiessuggested: Noneanti-IgGsuggested: (Vector Laboratories Cat# BA-8000, RRID:AB_2336140)a…SciScore for 10.1101/2020.07.01.20144295: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding Laboratory analysis was blinded to PCR status and SARS-CoV-2-specific-antibody results were reported as positive, negative or equivocal. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Antibodies Sentences Resources Mouse monoclonal anti-human HRP-conjugated antibodies (anti-IgG clone R-10 1:8000, anti-IgA MG4.156 1:4000 and anti-IgM AF6 1:2000; clones were generated at the University of Birmingham, available from Abingdon Health, UK) were diluted in 0.1% PBS-T. anti-human HRP-conjugated antibodiessuggested: Noneanti-IgGsuggested: (Vector Laboratories Cat# BA-8000, RRID:AB_2336140)anti-IgAsuggested: (LSBio (LifeSpan Cat# LS-C68423-2000, RRID:AB_10634906)anti-IgM AF6suggested: NoneSoftware and Algorithms Sentences Resources Statistical analysis: Statistical analyses were performed using Prism (version 8, GraphPad, USA). Prismsuggested: (PRISM, RRID:SCR_005375)GraphPadsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:A current limitation in antibody assays is the necessity for venepuncture by skilled phlebotomists. The use of DBS overcomes this limitation and introduces the opportunity for wider population level sampling and improved surveillance in those groups shielding from the infection. For example, DBS could be delivered using postal services6 to patients with chronic conditions, the immunocompromised and the elderly; groups which have been disproportionately affected by COVID-1914. Furthermore, the DBS method is simple and inexpensive5,6 which could enhance sampling in LMICs, amongst groups where venepuncture is culturally unacceptable or, in a geographically dispersed populous15.
Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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